%0 Journal Article
%T DNA条形码试剂盒检测技术在墨天牛鉴定中的应用
%A 陈梦义
%A 徐
%A 梅
%A 钱
%A 路
%A 安榆林
%A 郝德君
%J 南京林业大学学报(自然科学版)
%D 2015
%R 10.3969/j.issn.1000-2006.2015.06.002
%X 为了对进境口岸截获的俄罗斯木材中的墨天牛属(非中国种)害虫进行准确、快速鉴定,笔者利用DNA 条码试剂盒检测技术,基于墨天牛线粒体COⅠ(线粒体细胞色素氧化酶亚基I)基因设计一对巢式引物,对截获墨天牛属中的8 个种进行COⅠ扩增,将扩增序列上传至有害生物信息网站,依据碱基位点在不同种间的排列和组成的差异,获得不同种在相应位点上的特有碱基,即标记性碱基,以此作为区分墨天牛种的依据。通过这种墨天牛属DNA 条码试剂盒检测技术,可为口岸检疫鉴定提供有效的技术手段。</br>In order to rapidly and accurately identify the Monochamus of wood from Russian, we employed DNA barcode detection kit technology and designed a pair of nested primers based on mitochondrial COⅠ gene(mitochondrial cytochrome oxidase subunit I). Furthermore, COⅠ gene sequence of eight Monocharnus species was amplificated, and uploaded to pest information site. According to differences of arrangement and composition among these species at base sites, species unique bases was got in the corresponding sites, which is named specific site to distinguish this species. In this way, we developed the DNA barcoding identification kit technology to Monochamus species, which will provide a new way for port quarantine identification
%K 墨天牛属
%K COⅠ基因
%K DNA条形码试剂盒
%K 标记性碱基&lt
%K /br&gt
%K Monochamus
%K COⅠ gene
%K DNA barcoding identification kit technology
%K specific site
%K quarantine
%U http://nldxb.njfu.edu.cn/oa/darticle.aspx?type=view&id=201506003