%0 Journal Article
%T 雷公藤红素对白血病HL-60和Jurkat细胞增殖及凋亡的影响
%A 张晓玲
%A 李爱萍
%A 环飞
%A 秦珩
%A 段金廒
%J 中国药科大学学报
%D 2015
%X 探讨雷公藤红素对人急性髓性白血病HL-60细胞、急性T淋巴细胞白血病Jurkat细胞增殖及凋亡的影响。采用MTT法、AnnexinV-FITC/PI双染法、PI染色法、透射电镜观察不同浓度雷公藤红素作用于两种细胞后对其生长增殖、凋亡、周期及形态等方面的影响。结果表明雷公藤红素能显著抑制HL-60细胞、Jurkat细胞的增殖，降低其存活率。给药24 h后，半抑制浓度(IC50)分别为(0.46±1.05)μmol/L和(0.88±1.13)μmol/L。以剂量依赖方式诱导两种细胞凋亡，细胞周期分布G1期比例增加，S期比例降低(P<；0.05)，且伴有典型的细胞凋亡形态学改变。雷公藤红素能显著抑制HL-60细胞、Jurkat细胞的生长增殖，并诱导细胞凋亡。</br>To determine the effects of celastrol on the proliferation and apoptosis of cell lines HL-60 and Jurkat in human leukemia. Human leukemia cell lines were treated with celastrol at different concentrations. The inhibitory rate of cell proliferation was detected by MTT assay; the apoptosis rate was detected by AnnexinV-FITC/PI double staining; cell cycle was observed by PI staining; cell morphology was observed by transmission electron microscope(TEM). Cell proliferation was inhibited by celastrol, with IC50 of(0. 46±1. 05)μmol/L and(0. 88±1. 13)μmol/L at 24 h. Celastrol induced cell apoptosis in a dose-dependent manner. The cell cycle distribution of G1 phase rate increased, S phase rate decreased(P< 0. 05)with typical cell apoptosis-induced morphological changes. Results showed that celastrol could significantly inhibit cell proliferation and induce apoptosis in human leukemia cell lines of HL-60 and Jurkat
%K 雷公藤红素 HL-60细胞 Jurkat细胞 凋亡&lt
%K /br&gt
%K celastrol HL-60 cell Jurkat cell apoptosis
%U http://www.zgykdxxb.cn/jcpu/ch/reader/view_abstract.aspx?file_no=20150113&flag=1