%0 Journal Article
%T 牛奶中残留麻保沙星的微生物学检测方法
%A 陈秀金
%A 任国艳
%A 许凯旋
%A 崔璨
%A 张永丽
%A 侯玉泽
%A 胥传来
%A 李根
%J 食品与生物技术学报
%D 2018
%R 10.3969/j.issn.1673-1689.2018.01.018
%X 建立牛奶中残留麻保沙星的微生物学检测方法。试验以金黄色葡萄球菌为敏感菌，采用滤纸片法进行测定，以牛奶为样设计了添加回收试验以证实该方法的有效性。磷酸盐缓冲溶液和牛奶基质麻保沙星标准曲线的工作范围是0.1~1.6 μg/mL，最低检测限为 0.1 μg/mL。对纯牛奶样的3种添加水平，平均回收率范围在77.3%~93.3%之间，变异系数小于7.26%，该方法可以用于牛奶中麻保沙星残留的检测。</br>To establish a microbiological method for determination of marbofloxacin residues in milk. Marbofloxacin residues in milk was tested using filter paper method based on Staphylococcus selected as a sensitive bacteria，and then the recoveries were performed on milk samples to verify the feasibility of this method. Results：the data showed that the linear range for mornitoring marbofloxacin was from 0.1 μg/mL to 1.6 μg/mL based on phosphate buffer solution and milk matrix. The minimum limit detections of marbofloxacin in phosphate buffer solution and milk matrix were 0.1 μg/mL. The average recovery rates were in the range of 77.3%~93.3% and variation coefficient were less than 7.26% for three spiked levels of Erie milk samples. The obtained method can be feasible to mornitoring marbofloxacin residues in milk
%K 麻保沙星 牛奶 滤纸片法 残留检测&lt
%K /br&gt
%K marbofloxacin
%K milk
%K filter paper method
%K residue determination
%U http://spyswjs.cnjournals.com/spyswjs/ch/reader/view_abstract.aspx?file_no=201801018&flag=1