%0 Journal Article
%T Biofield Energy Enriched Vitamin D3 versus Vitamin D3 in Preventing Fractures and Bone Loss Using MG-63 Cells
%A Lorraine Marie Hachfeld
%A Mahendra Kumar Trivedi
%A Alice Branton
%A Dahryn Trivedi
%A Gopal Nayak
%A Mayank Gangwar
%A Snehasis Jana
%J Orthopedic Research Online Journal
%P 1-7
%D 2018
%R 10.31031/OPROJ.2018.03.000572
%X Bone is a dynamic tissue, which continually adapting its structure during the development process. The aim of the study was to investigate the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization using MG-63 cells. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Lorraine Marie Hachfeld and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability was more than 79% with a safe and nontoxic profile on MG-63 cell line. The level of ALP was increased by 400%, 84%, and 179.4% at 0.1, 1, and 10¦Ìg/mL, respectively in the UT-DMEM BT-TI, while 314.3% at 10¦Ìg/mL in the BT-DMEM UT-TI group as compared with the untreated test item and DMEM group. BT-DMEM BT-TI group showed an increased ALP level by 128% and 60.3% at 1 and 10¦Ìg/mL, respectively. Collagen was significantly increased by 180% and 128.1% at 0.1 and 1¦Ìg/mL, respectively in the UT-DMEM BT-TI group, while 84.4% and 69.7% at 1 and 10¦Ìg/mL, respectively in BT-DMEM UT-TI group as compared with the untreated group. Furthermore, BT-DMEM BT-TI group showed a significant increased collagen level by 123.4% and 184.8% at 1 and 10 ¦Ìg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 385.1% at 0.1¦Ìg/mL in the UT-DMEM BT-TI group, while 53.2%, 30.2%, and 163.9% at 0.1, 1, and 10¦Ìg/mL, respectively in BT-DMEM UT-TI group as compared with the untreated group. In addition, BT-DMEM BT-TI group showed a significant increased bone mineralization by 283%, 65.1%, and 197.3% at 0.1, 1, and 10¦Ìg/mL, respectively as compared with the untreated group. The experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. It regulates the osteoblast function, improves the bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.
%K Biofield healing
%K Bone mass
%K Osteosarcoma cells
%K Calcium absorption
%K Vitamin D
%K Bone mineralization
%U https://crimsonpublishers.com/oproj/pdf/OPROJ.000572.pdf