%0 Journal Article
%T MiR-373-3p Promotes Invasion and Metastasis of Lung Adenocarcinoma Cells
%A Aibing WU
%A Jinmei LI
%A Kunpeng WU
%A Yanli MO
%A Yiping LUO
%A Haiyin YE
%A Xiang SHEN
%A Shujun LI
%A Yahai LIANG
%A Meilian LIU
%A Zhixiong YANG
%J Öйú·Î°©ÔÓÖ¾
%D 2015
%R : 10.3779/j.issn.1009-3419.2015.07.07
%X Background and objective Lung cancer is the leading cause of cancer-related deaths worldwide, and metastasis is the major cause of death in lung cancer patients. MiR-373 is closely associated with invasion and metastasis in other tumor cells. This study explored the expression of miR-373-3p in non-small cell lung cancer (NSCLC) and its effect on the invasive and metastatic capabilities of lung adenocarcinoma cells, as well as their mechanisms of action. Methods The expression of miR-373-3p in NSCLC tissues and lung adenocarcinoma cell lines was detected by quantitative reverse transcription polymerase chain reaction. The roles of miR-373-3p in regulating lung adenocarcinoma cell invasion and metastatic properties were analyzed with miR-373-3p mimic/inhibitor-transfected cells via Transwell chamber assay. Matrix metalloproteinase MMP-9 and MMP-14 protein levels were detected by Western blot in lung cancer cells after transfection. Results MiR-373-3p was upregulated in 51 NSCLC tissues and 5 NSCLC cell lines. Gain-of-function and loss-of-function studies showed that overexpression of miR-373-3p promoted H1299 cell migration and invasion, which resulted in upregulation of MMP-9 and MMP-14. By contrast, miR-373-3p knockdown inhibited these processes in A549 cells and downregulated the expression of MMP-9 and MMP-14. Conclusion Our results demonstrated that miR-373-3p participated in the invasion and metastasis of lung adenocarcinoma cells, partly by upregulation of MMP-9 and MMP-14.
%K Lung neoplasms
%K MiR-373
%K MMP-9
%K MMP-14
%K Invasion
%K Metastasis
%U http://www.lungca.org/index.php?journal=01&page=article&op=view&path%5B%5D=10.3779%2Fj.issn.1009-3419.2015.07.07