%0 Journal Article
%T 不同寄主来源轮枝孢菌的交互致病性
%A 张园园
%A 张贵
%A 张键
%A 张光
%A 周洪友
%A 赵君
%J 浙江大学学报(农业与生命科学版)
%D 2017
%R 10.3785/j.issn.1008-9209.2016.09.281
%X 为了明确不同寄主来源的轮枝孢菌是否存在交互致病性，对来源于向日葵、棉花、茄子、生菜和马铃薯5种不同寄主植物上的10株轮枝孢菌的生物学特性，如菌落形态、生长速度、生理小种、交配型和致病力分化进行研究，并利用大丽轮枝孢菌不同生理小种和交配型的特异引物对供试大丽轮枝孢菌菌株进行聚合酶链式反应（polymerase chain reaction, PCR）检测。结果表明，不同寄主来源的轮枝孢菌在菌落形态和生长速度上存在显著差异。PCR检测结果表明：除了来自生菜的大丽轮枝孢菌Ls16-1为1号生理小种外，其余8株不同寄主来源的大丽轮枝孢菌均为2号生理小种；所有供试大丽轮枝孢菌菌株的交配型均为MAT1-2-1型。此外，采用纸钵撕底蘸根法将不同寄主来源的轮枝孢菌分别接种向日葵、棉花、茄子和马铃薯。结果显示，不同寄主来源的菌株对上述不同寄主均表现出不同程度的致病力，但都对自身分离寄主的致病性表现最强。综上表明，不同寄主来源的轮枝孢菌对不同寄主存在交互致病性，但均对其分离寄主的致病性表现最强。 通讯作者: 赵君(http://orcid.org/0000-0001-7207-9289) E-mail: zhaojun@imau.edu.cn</br>Abstract: Verticillium spp. is a group of soil-borne pathogen causing a yellow wilt disease on different kinds of hosts, such as cottons, potatoes, sunflowers and egg-plants, etc. So far, Verticillium dahliae and Verticillium alboatrum have been the most dominant species isolated from different diseased hosts. In this study, V. dahliae from sunflower, cotton, eggplant, lettuce and potato and V. alboatrum from potato were selected to study the colonial morphology, growth speed, and pathogenic specificity. Their race and mating types were also identified by specific primers. The results showed that variable colonial morphology and growth speed were observed in all the tested isolates. Four different kinds of colonial morphologies were classified based on the amount of aerial mycelium and the number of microsclerotia. However, the difference on the morphology of conidiophores and conidia was not obvious among all the tested isolates. The growth speed was also variable after 10 d of culture on potato D-glucose agar (PDA) medium. The strain Ls17-6 from lettuce grew the fastest, while Hn-1 from cotton grew the slowest. The difference of growth speed was also compared with the other two isolates. All the tested isolates were characterized as race 2, except for Ls16-1 isolated from lettuce as race 1; the mating type of all the tested strains was MAT1-2-1. The pathogenic specificity was studied using root dipping inoculation way. Twenty-one days post-inoculation (dpi), the pathogenicity of isolates always showed the highest virulence on its own host. For example, Sx5 which was isolated from sunflower showed the highest disease index of 52.50, followed by the isolate V991 from cotton with the disease index of 41.25. The same result was also obtained on cotton. For example, the isolate V991 showed the highest pathogenicity, and Icd3-33 also from cotton listed in the second position among all the tested isolates. In conclusion, the pathogenicity of Verticillium spp. on the different hosts is variable, but shows the strongest pathogenicity in the
%U http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2016.09.281