%0 Journal Article
%T R9-FOXM1(1-234aa)重组蛋白批量纯化及其抑瘤效应研究
%A 谭拥军
%A 余景卫
%A 陈燕
%A 向勤
%A 谭桂湘
%J 湖南大学学报(自然科学版)
%D 2017
%X 通过基因工程构建重组表达质粒pET15b-R9-FOXM1(1-234aa)，转化大肠杆菌建立构建表达R9-FOXM1(1-234aa)的菌株.采用原核表达系统和His-tag亲和纯化手段，规模化制备纯化穿膜肽R9-FOXM1(1-234aa)，获得的蛋白的纯度达到90%以上.用R9-FOXM1(1-234aa)穿膜肽处理不同的肿瘤细胞，通过MTT实验研究其细胞效应.结果显示：当R9-FOXM1(1-234aa)穿膜肽的浓度达到2 mM时，肿瘤细胞的死亡率为50%左右.实验表明穿膜肽R9-FOXM1(1-234aa)抑制不同肿瘤细胞的生长，有可能成为治疗肿瘤的潜在蛋白类药物.</br>A recombinant protein expression vector pET15b-R9-FOXM1(1-234aa) was constructed and transformed to E. coli in order to generate a strain expressing R9-FOXM1(1-234aa). The recombinant protein R9-FOXM1 (1-234aa) (R9-FOXM1(1-234aa)) was isolated at a large scale through His-tag affinity chromatography. The purity of the purified protein reached 90%. Moreover, MTT assay was used to test the effect of R9-FOXM1(1-234aa) on cells, and the test results showed that R9-FOXM1(1-234aa) caused the cell death of different types of cancer cells with a half lethal dose around 2 mm。 The results also demonstrated that R9-FOXM1(1-234aa) suppressed the proliferation of cancer cells and may be considered as a potential angent for anti-cancer in the future.
%K 多聚精氨酸 细胞穿膜肽 FOXM1 肿瘤治疗&lt
%K /br&gt
%K arginine-rich cell-penetrating peptides FOXM1 tumor therapy
%U http://hdxbzkb.cnjournals.net/ch/reader/view_abstract.aspx?file_no=20171218&flag=1