%0 Journal Article
%T 水稻bHLH转录因子Os11g39000的功能研究
%A 刘选明
%A 彭玉冲
%A 杨远柱
%A 李懿星
%A 夏妙林
%A 王文文
%A 林建中
%J 湖南大学学报(自然科学版)
%D 2016
%X 水稻Os11g39000 为非典型的bHLH家族成员，其功能尚不清楚.酵母双杂交实验表明，转录因子Os11g39000可以形成同源蛋白聚合体.随机结合位点筛选实验表明，转录因子Os11g39000可以与DNA结合，并且其结合位点初步确定为TT/CG/CACC/GT/C.Os11g39000基因的组织表达模式分析发现，Os11g39000基因主要在叶片和根中表达，推测该基因可能在叶和根的发育调控中发挥作用.水培实验发现，该基因功能缺陷型转基因株系的根长显著短于野生型，表现出明显的根部发育缺陷，表明Os11g39000在水稻根的发育中起调控作用.Q-PCR分析进一步证明，功能缺陷型转基因植株体内生长素合成和信号转导相关基因表达量显著上升，表明转录因子Os11g39000参与了水稻生长素的合成和信号转导的调控.</br>Os11g39000 is a member of atypical bHLH transcription factor family in rice, and its function remains unclear. Yeast two-hybrid experiments revealed that the transcription factor Os11g39000 could form a homologous protein polymer. Random binding site selection demonstrated that Os11g39000 could bind to DNA, and its binding site was preliminarily identified as TT/CG/CACC/GT/C. Expression pattern analysis discovered that the Os11g39000 gene was mainly expressed in leaves and roots, which was a speculation that Os11g39000 may play a role in the developmental regulation of leaves and roots. The hydroponic experiments showed that, compared with wild-type plants, the loss-of-function transgenic plants exhibited much shorter roots and obvious defect in root development, indicating that Os11g39000 was involved in the developmental regulation of roots. Q-PCR analysis further revealed that the transcriptional levels of auxin biosynthesis and signal transduction related genes increased significantly in loss-of-function transgenic plants. These results together demonstrated that the transcription factor Os11g39000 was involved in the regulation of auxin biosynthesis and signal transduction.
%K 水稻 bHLH转录因子 Os11g39000 生长素&lt
%K /br&gt
%K rice bHLH transcription factor Os11g39000 auxin
%U http://hdxbzkb.cnjournals.net/ch/reader/view_abstract.aspx?file_no=20160619&flag=1