%0 Journal Article
%T 聚天冬氨酸苄酯修饰的泊洛沙姆纳米粒制备和表征
%A 马丽芳
%A 张婷
%A 郭丽
%A 李瑶
%A 王晓姣
%J 工程科学与技术
%D 2016
%X 中文摘要: 本研究的目的是通过泊洛沙姆188引发L-天冬氨酸-β-苄酯-N-羧酸酐(BLA-NCA)开环聚合，合成一种新型的药物载体材料PBLA-POLO-PBLA嵌段共聚物。利用核磁共振（1H-NMR）、傅里叶变换红外光谱法（FTIR）和凝胶渗透色谱（GPC）对合成的聚合物结构和分子量进行表征；运用CCK-8法研究了聚合物材料对A549的细胞毒性。采用乳化溶剂蒸发法制备伐昔洛韦（VACV）聚合物纳米粒并对其理化性质进行了研究。动态光散射粒度仪（DLS）和透射电子显微镜（TEM）结果显示该载药纳米粒的尺寸为200nm左右, 具有圆球形态并且分散性能良好。用高效液相色谱法测得其载药量为4.36%，以透析法观察到载药纳米粒在磷酸缓释溶液中96h时释放达到70.22%，具有明显缓释作用。因此，PBLA-POLO-PBLA嵌段共聚物在纳米载药系统中作为药物载体有着良好的应用前景。</br>Abstract:The aim of the present study was designing a novel drug carrier system composed of the block copolymer PBLA-POLO-PBLA via ring-opening polymerization of the β-benzyl-Lasparate N-carboxyanhydride (BLA-NCA) monomer which was initiated by poloxamer 188. The polymers were characterized and confirmed by 1H-NMR, Fourier transform infrared assay (FTIR) and gel permeation chromatography (GPC). Then, studying with A549 cells to evaluate the material`s cytotoxicity showed that the copolymer was nontoxic. PBLA-POLO-PBLA nanoparticles loaded with Valaciclovir (VACV) were prepared by the emulsion-solvent evaporation method, and the physicochemical properties were also investigated afterwards. The transmission electron microscope (TEM) showed that the VACV-loaded nanoparticles had a sub-micron size with a spherical shape. The VACV-loaded nanoparticles (with a loading efficiency of 4.36%) showed a sustained release of VACV molecules (with 70.22% release in 96 h) which was determined by HPLC at 251 nm. These results suggested that PBLA-POLO-PBLA block copolymer was a promising nano-carrier in nanoparticle drug delivery systems.
%K 泊洛沙姆 聚天冬氨酸苄酯 嵌段共聚物 纳米粒 载体&lt
%K /br&gt
%K Poloxamer PBLA Block copolymers Nanoparticles Carrier
%U http://jsuese.ijournals.cn/jsuese_cn/ch/reader/view_abstract.aspx?file_no=201500841&flag=1