%0 Journal Article %T 阿魏植物精油对紫色杆菌群体感应的影响 %A 王锦利 %J 现代食品科技 %D 2016 %R 10.13982/j.mfst.1673-9078.2016.10.015 %X 本研究采用肉汤微量稀释法对阿魏精油的抗菌活性进行了研究，同时使用紫色杆菌作为细菌模型对阿魏精油的群体抑制剂活力进行了评估。结果表明：阿魏可作为与食品有关微生物和群体感应的抑制剂。阿魏精油的最小抑菌浓度(MIC)值为0.25 mg/mL，可以抑制所有被测细菌的生长。对于阪崎肠杆菌/大肠杆菌/鼠伤寒沙门菌/李斯特菌，其最小杀菌浓度(MBC)值分别为0.25、0.50、0.48、0.48 mg/mL。此外，含浓度为6.25和12.5 mg/mL的阿魏精油纸片显著的抑制紫色杆菌(Chromobacterium violaceum)色素的产生，但不影响紫色杆菌的生长。当浓度被提升到25 mg/mL，既抑制色素的产生也抑制细菌的生长。同样地，所有浓度的阿魏精油抑制了紫色杆菌素的产生，即使最低浓度的阿魏精油0.13 mg/mL，也使色素产物减少38%以上；浓度为0.13、0.50和1.0 mg/mL的阿魏精油不影响紫色杆菌的生存能力，只有最高浓度2.0 mg/mL抑制了细菌的生长，在培养后使细菌数量减少1.37 log CFU/mL。这些结果表明阿魏精油作为一种新型的群体感应抑制剂可有效影响紫色杆菌的群体感应机制。</br>The broth microdilution method was used to evaluate the antibacterial activity of Ferula essential oil (FEO), and the quorum sensing (QS) inhibitory activity of FEO was evaluated using Chromobacterium violaceum as a bacterial model. The results revealed that FEO could be used as a food antimicrobial agent and quorum sensing inhibitor. FEO showed a strong inhibitory effect on bacterial growth at minimum inhibitory concentration (MIC) of 0.25 mg/mL for all tested bacteria. Additionally, the minimum bactericidal concentration (MBC) values of FEO against Cronobacter sakazakii, Escherichia coli, Salmonella typhimurium, and Listeria monocytogenes were 0.25, 0.50, and 0.48 mg/mL, respectively. Additionally, the paper disks containing FEO at 6.25 and 12.5 mg/mL significantly inhibited the pigment produced by C. violaceum, but did not affect the growth of C. violaceum CVO26. However, when the concentration was increased to 25 mg/mL, both bacterial growth and the pigment production were inhibited. The FEO samples at all tested concentrations inhibited violacein production by C. violaceum ATCC 12472, and even FEO at the lowest concentration (0.13 mg/mL) reduced pigment production by more than 38%. In addition, the viability of C. violaceum was not affected when the concentrations of FEO samples were 0.13, 0.50, and 1.0 mg/mL, and only the FEO at the highest concentration (2.0 mg/mL) inhibited the bacterial growth and reduced bacterial counts by 1.37 log CFU/mL after incubation. These results show that FEO can effectively affect the quorum sensing system in Chromobacterium violaceum as a novel QS inhibitor. %K 阿魏精油 紫色杆菌 群体感应 抗菌活性< %K /br> %K Ferula essential oil Chromobacterium violaceum quorum sensing antibacterial activity %U http://www.xdspkj.cn/ch/reader/view_abstract.aspx?file_no=20161015&flag=1