%0 Journal Article
%T T细胞淋巴瘤长链非编码RNA <em>ST20-AS1</em>的表达及其生物学作用<br>Expression and biological effect of long non-coding RNA？ <em>ST20-AS1</em>？ in T-cell lymphoma
%A 孙漪峰 许彭鹏 赵维莅&lt
%A br&gt
%A SUN Yi-feng
%A XU Peng-peng
%A ZHAO Wei-li
%J 上海交通大学学报（医学版）
%D 2016
%R 10.3969/j.issn.1674-8115.2016.09.002
%X 目的·检测T细胞淋巴瘤患者长链非编码RNA（lncRNA）ST20-AS1的表达，并探索其对T细胞淋巴瘤细胞增殖和侵袭性的影响。方法·运用lncRNA芯片筛选出ST20-AS1作为研究的lncRNA，通过芯片数据发现ST20-AS1的高表达与NOTCH信号通路的关系；Real-time RT-PCR方法验证ST20-AS1在T细胞淋巴瘤患者中的表达水平，并验证其对NOTCH通路的激活作用；构建ST20-AS1稳定表达的T淋巴瘤Jurkat细胞株，进行细胞增殖和Transwell侵袭实验，观察细胞增殖和侵袭性的改变。结果·ST20-AS1在T淋巴瘤患者中呈高表达，ST20-AS1高表达患者的NOTCH通路被显著激活。在Jurkat细胞株中发现，ST20-AS1高表达活化NOTCH信号通路，增强细胞增殖和侵袭能力。利用siRNA敲低NOTCH1的表达后，ST20-AS1高表达细胞株的增殖和侵袭能力降低。结论·ST20-AS1激活NOTCH信号通路，促进淋巴瘤细胞的增殖和侵袭性。<br>： Objective·To measure the expression of ST20-AS1, a long non-coding RNA (lncRNA) in patients with T-cell lymphoma, and to explore its effects on the proliferation and invasion of T-cell lymphoma cells. Methods·Study lncRNA was screened using the lncRNA array. The association between high expression of ST20-AS1 and NOTCH signaling pathway was detected via the data from the lncRNA array. The expression level of ST20-AS1 in T-cell lymphoma patients was assessed by real-time RT-PCR and its activation effect on NOTCH signaling pathway was verified. ST20-AS1-overexpressing T-cell lymphoma cell line Jurkat was constructed and cell proliferation test and Transwell assay were performed to observe changes in cell proliferation and invasion. Results·ST20-AS1 was overexpressed and NOTCH signaling pathway was significantly activated in T-cell lymphoma patients. The overexpression of ST20-AS1 activated the NOTCH signaling pathway and promoted lymphoma cell proliferation and invasion in Jurkat. When NOTCH1 was knocked down by siRNA, cell proliferation and invasion was reduced in ST20-AS1-overexpressing cell line Jurkat. Conclusion·ST20-AS1 can activate the NOTCH signaling pathway and promote lymphoma cell proliferation and invasion
%K T细胞淋巴瘤
%K ST20-AS1
%K NOTCH信号通路
%K 细胞增殖
%K 细胞侵袭
%K &lt
%K br&gt
%K T-cell lymphoma
%K ST20-AS1
%K NOTCH signaling pathway
%K cell proliferation
%K cell invasion
%U http://xuebao.shsmu.edu.cn/CN/abstract/abstract11278.shtml