%0 Journal Article %T 循环CD34<sup>+</sup> 细胞数量与老年高血压的关系<br>Correlation between circulating CD34<sup>+</sup> level and elderly patients with hypertension？？ %A 黄黎莹 %A 沈培晓 %A 汪海娅< %A br> %A HUANG Li-ying %A SHEN Pei-xiao %A WANG Hai-ya %J 上海交通大学学报（医学版） %D 2016 %R 10.3969/j.issn.16748115.2016.03.018 %X 目的 探讨循环CD34+ 细胞水平与老年高血压关系，分析Ang-1/Tie-2 在内皮祖细胞的表达及其调控意义。方法 选择老年高血压患者59例为高血压组，正常健康者59例为对照组。RT-PCR法检测Ang-1/Tie-2 mRNA表达，应用流式细胞仪测定外周血CD34+/PMNC值。分析外周血CD34+/PMNC值与各临床指标的相关性，以及Ang-1和Tie-2 mRNA水平与CD34+/PMNC值的相关性。结果 高血压组外周血CD34+/PMNC值显著低于对照组（P=0.000）。高血压组外周血CD34+/PMNC值与收缩压呈负相关（r=-0.622,P=0.009），与舒张压、总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、空腹血糖、尿酸水平无相关性。高血压组内皮祖细胞Ang-1和Tie-2 mRNA水平显著低于对照组（P=0.000），与CD34+/PMNC值分别呈正相关（r=0.561,P=0.005；r=0.473,P=0.009）。结论 老年高血压患者的心血管病变可能与Ang-1和Tie-2水平的下调从而影响内皮祖细胞的修复功能有关。<br>： Objective To explore the correlation between circulating CD34+ cell level and elderly patients with hypertension and analyze Ang-1/Tie-2 expression in endothelial progenitor cells and its regulatory meaning. Methods Fiftynine elderly patients with hypertension and 59 healthy people were enrolled and assigned to the hypertension group and the control group, respectively. The mRNA level of Ang-1/Tie-2 was measured by RTPCR. Flow cytometry was used to detect the CD34+/PMNC value in peripheral blood. The correlations between CD34+/PMNC value in peripheral blood and clinical indexes and between Ang-1 and Tie-2 mRNA levels and CD34+/PMNC value were analyzed. Results The CD34+/PMNC value in peripheral blood of the hypertension group was significantly lower than that of the control group (P=0.000). The CD34+/PMNC value in peripheral blood of the hypertension group was negatively correlated to systolic pressure (r=-0.622; P=0.009), while was not correlated to diastolic pressure, total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol levels, fasting blood glucose and uric acid. The Ang-1 and Tie-2 mRNA levels in endothelial progenitor cells of the hypertension group were significantly lower than those in the control group (P=0.000) and were positively correlated to CD34+/PMNC value （r=0.561,P=0.005；r=0.473,P=0.009）. Conclusion Cardiovascular lesions in elderly patients with hypertension may be associated with the downregulation of Ang-1 and Tie-2 levels, which affects repair function of endothelial progenitor cells %K 老年高血压 %K 循环内皮祖细胞 %K CD34< %K sup> %K +< %K /sup> %K < %K br> %K elderly patients with hypertension %K circulating endothelial progenitor cells %K CD34< %K sup> %K +< %K /sup> %U http://xuebao.shsmu.edu.cn/CN/abstract/abstract11086.shtml