%0 Journal Article %T 高氧致SD大鼠年龄相关的肺损伤及可能机制<br>Mechanism of age-related hyperoxia-induced lung injury in SD rats %A 宁巍 %A 李静 %A 漆秀洁 %A 许峰 %A 方芳 < %A br> %A NING Wei %A LI Jing %A QI Xiu-jie %A XU Feng %A FANG Fang %J 上海交通大学学报（医学版） %D 2016 %R 10.3969/j.issn.1674-8115.2016.11.005 %X 目的 ·观察新生鼠和幼鼠高氧肺损伤的异同，并初步探索Rho/Rock信号通路在年龄相关高氧肺损伤中的作用。方法 ·将新生SD大鼠（新生鼠）与3周龄SD大鼠（幼鼠）分为新生鼠空气组、新生鼠高氧组、幼鼠空气组、幼鼠高氧组，建立动物模型直至第14日，统计生存率、体质量变化，取SD大鼠肺组织行病理学检查、损伤程度评分、羟脯氨酸含量测定、SOD与MDA检测，以及ROCK1、p-MYPT1、MYPT1表达的检测。结果 ·①新生鼠高氧组较新生鼠空气组、幼鼠空气组、幼鼠高氧组生存率明显降低，幼鼠空气组与高氧组生存率无明显差异；新生鼠高氧组较空气组、幼鼠高氧组较空气组体质量增长缓慢，且新生鼠高氧组较幼鼠高氧组体质量增长更为缓慢。②新生鼠高氧组、幼鼠高氧组出现肺组织损伤及纤维化改变，且新生鼠较幼鼠更为严重。③新生鼠高氧组较空气组、幼鼠高氧组较空气组MDA含量升高，SOD活力降低；与幼鼠高氧组相比，新生鼠高氧组SOD活力基础值较低且高氧后降低更为明显。④新生鼠高氧组较空气组、幼鼠高氧组较空气组肺组织ROCK1蛋白表达有上升趋势，p-MYPT1蛋白表达增强，且新生鼠高氧组较幼鼠高氧组p-MYPT1蛋白表达增强更为明显。结论 ·高氧肺损伤具有年龄依赖性，新生鼠对高氧耐受力明显弱于幼鼠，与其自身抗氧化能力弱有关；Rho/Rock信号通路活化程度的不同可能在年龄相关高氧肺损伤中发挥重要作用。<br>： Objective · To observe the similarities and differences in neonatal rats and immature rats with hyperoxia-induced lung injury and to preliminary explore the role of Rho/Rock signaling pathway in age-related hyperoxia-induced lung injury. Methods · Sprague-Dawley neonatal rats and immature rats aged 3 weeks were randomly assigned to the neonatal air group, the neonatal hyperoxia group, the immature air group, and the immature hyperoxia group. The changes in survival rate and body mass were statistically observed form the construction of animal model till 14 d. Lung tissues of SD rats were harvested. Pathological examination and injury scoring were performed and hydroxyproline, SOD, MDA, ROCK1, p-MYPT1, and MYPT1 were examined. Results · ① The neonatal hyperoxia group had a significantly lower survival rate than the neonatal air group, the immature air group, and the immature hyperoxia group. There was no significant difference in survival rate between the immature air group and the immature hyperoxia group. The body weight increased slower in the neonatal and immature hyperoxia groups than in the neonatal and immature air groups. The body weight increased slower in the neonatal hyperoxia group than in the immature air group. ② The neonatal hyperoxia group and immature hyperoxia group developed lung injury and fibrosis. The neonatal hyperoxia group was more serious than the immature hyperoxia group. ③ The neonatal and immature hyperoxia groups had higher MDA level and lower SOD activity than the neonatal and immature air groups. The neonatal hyperoxia group had lower SOD activity than the immature hyperoxia group. The SOD activity was significantly decreased after hyperoxia treatment. ④ The neonatal and immature hyperoxia groups had higher ROCK1 and p-MYPT1 expressions than the neonatal and immature air groups. The neonatal hyperoxia group had higher p-MYPT1 expression than the immature hyperoxia group. Conclusion · Hyperoxia-induced lung injury is age-dependent. %K 高氧 %K 肺损伤 %K 年龄相关 %K 超氧化物歧化酶 %K 氧化应激 %K Rho/Rock信号通路 %K < %K br> %K hyperoxia %K lung injury %K age-related %K SOD %K oxidative stress %K Rho/Rock signaling pathway %U http://xuebao.shsmu.edu.cn/CN/abstract/abstract11344.shtml