%0 Journal Article
%T 三氧化二砷对肝细胞癌 HepG2细胞系作用效果的代谢组学研究<br>Metabolomics study of the effect of arsenic trioxide on hepatocellular carcinoma cell line HepG2
%A 黄巾帼
%A 张海南
%A 陈子卿
%A 杨丽娜
%A 郭书娟
%A 陶生策 &lt
%A br&gt
%A HUANG Jin-guo
%A ZHANG Hai-nan
%A CHEN Zi-qing
%A YANG Li-na
%A GUO Shu-juan
%A TAO Sheng-ce
%J 上海交通大学学报（医学版）
%D 2018
%R 10.3969/j.issn.1674-8115.2018.10.002
%X 目的 ·从代谢组学的角度揭示三氧化二砷（ arsenic trioxide，ATO）对肝细胞癌（ hepatocellular carcinoma，HCC）的作用机制。方法 ·分别取 ATO处理 12、24和 36 h的肝细胞癌 HepG2细胞系，通过气相色谱 /质谱联用和液相色谱 /串联质谱联用对其代谢物谱进行全局性分析。结果 ·共检测到 307种代谢物。生物信息学分析表明，糖基化和单碳代谢是主要受影响的细胞功能。其他细胞功能或途径如线粒体脂肪酸 β-氧化、三羧酸循环、戊糖磷酸途径和谷胱甘肽代谢等也受到影响。结论 · ATO通过影响 HepG2细胞主要的代谢途径，抑制细胞生长和迁移，并加剧氧化应激来诱导癌细胞凋亡。<br>：Objective · To reveal the mechanism of the effect of arsenic trioxide (ATO) on hepatocellular carcinoma (HCC) cells through metabolomics study. Methods · HCC cell line HepG2 was treated with ATO for 12 h, 24 h and 36 h respectively. The global metabolite profiles were monitoredmetabolomics analysis using GC/MS and LC/MS/MS. Results · A total of 307 certified metabolites were detected. Bioinformatics analysis showed that glycosylation and one-carbon metabolism were the main cellular functions that have been affected. Other affected cellular functions/pathways included mitochondrial function fatty acid β-oxidation, TCA cycle, pentose phosphate pathway (PPP) and glutathione metabolism. Conclusion · ATO treatment affects the main metabolomic pathway of HepG2, and inhibits cell growth and migration, then exacerbates oxidative stress to induce cancer cell apoptosis
%K 砷剂
%K 三氧化二砷
%K 肝细胞癌
%K HepG2
%K 代谢组学
%K &lt
%K br&gt
%K arsenic
%K arsenic trioxide
%K hepatocellular carcinoma
%K HepG2
%K metabolomics
%U http://xuebao.shsmu.edu.cn/CN/abstract/abstract12041.shtml