%0 Journal Article
%T 木霉菌REMI突变菌株在降解敌敌畏中的作用</br>Dichlorvos Degradation by REMI Mutants of Trichoderma koningii
%A 元旭
%A 菅丽萍
%A 陈捷
%J 上海交通大学学报农业科学版
%D 2016
%X 本研究对木霉菌T30野生株和限制性内切酶介导的DNA整合技术(REMI)构建的突变株进行降解敌敌畏能力和最佳条件的研究。结果表明,野生株和几个REMI突变株均能降解有机磷农药敌敌畏,但以突变株TK-3降解活性最高,其降解效率达98％。TK-3突变株降解有机磷农药效率取决于葡萄糖剂量、敌敌畏初始浓度、初始pH等。突变株降解敌敌畏的葡萄糖最佳浓度为1 000 μg/mL,最适pH为7.0,敌敌畏浓度500 μg/mL。敌敌畏浓度超过1 000 μg/mL则会抑制木霉菌降解能力。</br>The dichlorvos degradation abilities of Trichoderma koningii mutants obtained by REMI (restriction enzyme mediated integration) and the optimal conditions for effective degradation were studied.Results showed both the wild type (T30) and mutants (TK-1,TK-2,TK-3,TK-5,TK-7,TK-8,TK-21,TK-38,TK-53,TK-30,TK-42)were able to degrade dichlorvos.TK-3 could reach 98% dichlorvos decomposition, which is the most efficient among mutants.Dichlorvos degradation rate was closely associated with glucose, dichlorvos initial concentration and pH of the medium.It was found that glucose at 1 000 μg/mL,pH 7.0 and dichlorvos at 500 μg/mL were the optimal conditions for effectively degrading dichlorvos by the mutant TK-3.The degradation efficiency of TK-3 would decline if dichlorvos concentration exceeded 1 000 μg/mL.
%K 康氏木霉 限制性内切酶介导基因合技术(REMI) 敌敌畏 降解&lt
%K /br&gt
%K Trichoderma koningii restriction enzyme mediated integration (REMI) dichlorvos degradation
%U http://shjtdxnxb.cnjournals.com/ch/reader/view_abstract.aspx?file_no=20160115&flag=1