%0 Journal Article
%T 白细胞介素-32与妊娠期糖尿病的相关性
%A 于志艳
%A 黄新梅
%A 管军华
%A 张瑞
%A 杨敏
%A 吴跃跃
%A 查兵兵
%A 刘军△
%J 复旦大学（医学报）
%D 2018
%X </br>Objective To investigate the concentration of interleukin-32 (IL-32) in serum,and the expression of IL-32 mRNA in placenta,subcutaneous fat and great epiploon adipose tissue,and explore the relationship between IL-32 and gestational diabetes mellitus (GDM).Methods The concentrations of serum IL-32 in 42 GDM (GDM group) and 38 non-GDM (control group) pregnant women were examined by enzyme linked immunosorbent assay (ELISA).The level of the IL-32mRNA in the placenta,subcutaneous fat and great epiploon adipose tissue was examined by quantitative real-time reverse-transcription polymerase chain reaction (RT-qPCR).The expression levels of IL-32 protein in the placenta and umbilical cord were measured by immunohistochemical staining (IHC) and analyzed by ImagePro Plus 9.0.Results The serum level of IL-32 in the GDM group was significantly higher than that in the control group ［(129.3±5.78) pg/mL vs.(105.6±8.61) pg/mL,P<0.05］.The level of IL-32 mRNA was increased 1.32 and 1.66 fold in the placenta (P<0.05) and the great epiploon adipose tissue (P<0.05) from GDM women,compared with that from control group.No significant difference was found in the levels of IL-32mRNA in the subcutaneous adipose tissue between the two groups ［(3.78±0.53) vs.(3.61±0.35),P>0.05］.The expression of IL-32 in the placenta from GDM group was 1.27 times higher than that from control group (P <0.05).The IL-32 in the umbilical artery and umbilical vein of GDM group increased by 1.30 and 1.32 (P<0.05).But there was no significant difference of IL-32 expression in the umbilical interstitial between the two groups.Conclusions Overexpression of IL-32 in the serum and tissue may be involved in the pathogenesis of GDM.
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%K interleukin-32 diabetes mellitus gestation placenta umbilical cord adipose tissue
%U http://jms.fudan.edu.cn/CN/abstract/abstract1570.shtml