%0 Journal Article
%T 有机碳源对SNAD工艺脱氮性能及微生物种群结构的影响
%A 李冬
%A 何永平
%A 张肖静
%A 梁瑜海
%A 张玉龙
%A 范丹
%A 张杰
%J 哈尔滨工业大学学报
%D 2016
%R 10.11918/j.issn.0367-6234.2016.02.012
%X 为考察不同有机碳源质量浓度对亚硝化的全程自养脱氮工艺（SNAD）脱氮性能的影响，将该工艺应用到生活污水的处理中，采用MBR反应器，以葡萄糖作为有机物来源，通过逐步增大COD来实现，并运用PCR-DGGE技术研究了微生物种群结构的变化.反应器运行结果和DGGE图谱分析表明：碳氮比为0~2时，COD的增加不会抑制AOB和Anammox菌，AOB和Anammox菌的菌属种类不受影响，反而通过反硝化作用提高氮去除负荷.总氮去除率和氮去除负荷分别为67%和0.34 kg/（m3·d）左右.碳氮比为3~4及生活污水运行条件下，Anammox菌不受影响，AOB的活性受到抑制，菌属种类减少，脱氮效率下降.生活污水运行阶段，总氮去除率和氮去除负荷平均分别为73%和0.17 kg/（m3·d）.Nitrosomonas和Candidatus Kuenenia stuttgartiensis一直是反应器内的优势菌属，共同完成脱氮过程.</br>Effect of different organic substrate concentration on nitrogen removal in SNAD and the application of this process to treat domestic wastewater were investigated in a MBR, with gradually increased glucose as a source of organic matter. Besides, changes of microbial communities were observed by PCR-DGGE techniques. Experimental results and DGGE profiles showed that the increase of organic carbon concentration did not inhibited the activity and category species of AOB and Anammox bacteria when the C/N ration was ranged 0 to 2.0. Meanwhile, a significantly improvement of nitrogen removal was observed via effective denitrification, with a total nitrogen removal efficiency of 67% and nitrogen removal rate of 0.34 kg/(m3·d), respectively. Under the C/N of 3-4 condition, the anammox bacteria in the system changed insignificantly, but AOB was inhibited and the category species reduced, resulting a decreasing of nitrogen removal efficiency.Total nitrogen removal efficiency and nitrogen removal rate were about 73% and 0.17 kg/(m3·d), respectively.Nitrosomonas and Candidatus Kuenenia stuttgartiensis were the predominant microorganisms in the SNAD reactor and played a major role in autotrophic nitrogen removal.
%K 同步亚硝化厌氧氨氧化和反硝化
%K 有机物
%K 生活污水
%K 聚合酶链式反应-变性梯度凝胶电泳
%K 微生物&lt
%K /br&gt
%K SNAD
%K organic
%K carbon
%K domestic
%K wastewater
%K PCR-DGGE
%K microbes
%U http://hit.alljournals.cn/hitxb_cn/ch/reader/view_abstract.aspx?file_no=20160212&flag=1