%0 Journal Article
%T Inhibition of Autophagy Attenuated Cell Damage after OGD/R in SH-SY5Y Cells by Down-Regulating AMPK-Mediated Autophagy Signaling Pathway
%A Yanxue Zhang
%A Hongyun Li
%J Open Access Library Journal
%V 6
%N 2
%P 1-12
%@ 2333-9721
%D 2019
%I Open Access Library
%R 10.4236/oalib.1105240
%X <div style="text-align:justify;">
	<span style="line-height:1.5;">Background: Activation of autophagy becomes a new therapeutic target for the treatment of stroke. The aim of this study was to observe the role of autophagy in the model of glucose-oxygen deprivation reoxygenation (OGD/R) in SH-SY5Y cells by interfering with AMPK-mediated autophagy signaling pathway. Methods: The effects of autophagy on OGD/R injury in SH-SY5Y cells were investigate</span><span style="line-height:1.5;">d by evaluating cell viability and morphologic change using Cell Counting Kit-8 (CCK-8) and inverted microscop, respectively. To investigate whether autophagy played a role via the AMPK-mTOR signaling pathway, the levels of phospho-AMPK, phos-pho-mTOR, ULK1, microtubule-associated protein 1 light chain 3 (LC3) and Beclin 1 were detected using Western blot. In order to detect changes in autophagic flow after OGD/R, autophagic flux (number of autophagosomes and autophagosomes) was detected by tandem stably expressed a tandem GFP-mRFP-LC3 construct. Results: Autophagy was low in the control group. After OGD/R, the expression of autophagy-related proteins LC3 and Beclin 1 and autophagic flux increased, phospho-AMPK and ULK1 expression increased and phospho-mTOR expression decreased, meanwhile abnormal cell morphological changes increased significantly and cell viability decreased. Inhibition of AMPK activity, the levels of phospho-mTOR, ULK1, LC3, Beclin1 and the expression of autophagic flux were opposite to those of the model group; cell abnormal morphology and structure improved, and cell viability increased. Conclusion: Inhibition of autophagy can attenuate cell damage after OGD/R in SH-SY5Y cells by down-regulating AMPK-mediated autophagy signaling pathway.</span>
</div>
%K Autophagy
%K OGD/R
%K AMPK
%K SH-SY5Y Cells
%U http://www.oalib.com/paper/5304034