%0 Journal Article
%T Cyanidine-3-O-Galactoside Enriched <i>Aronia melanocarpa</i> Extract Inhibits Adipogenesis  and Lipogenesis via Down-Regulation of Adipogenic Transcription Factors and Their Target Genes in 3T3-L1 Cells
%A Su-Min Lim
%A Jae In Jung
%A Nam Young Kim
%A Jung-Shik Bae
%A Hyun Sook Lee
%A Eun Ji Kim
%J Food and Nutrition Sciences
%P 128-147
%@ 2157-9458
%D 2019
%I Scientific Research Publishing
%R 10.4236/fns.2019.102011
%X <em>Aronia melamocarpa </em>(AM) is a rich source of anthocyanins, which are known to help prevent obesity. The cyanidine-3-O-galactoside enriched AM extract (AM-Ex) containing more cyanidine-3-O-galactoside than conventional AM extract was recently developed. The objective of this study was to examine the effect of AM-Ex on adipogenesis and its action mechanisms in vitro using 3T3-L1 adipocytes. To examine the anti-obesity effect of AM-Ex, 3T3-L1 cells were induced adipocyte differentiation and incubated with various concentration of AM-Ex. Lipid accumulation, cellular triglyceride content, mRNA expression of transcription factors and adipogenic genes were analyzed. Treatment with 100 - 400 ¦Ìg/mL of AM-Ex resulted in a dose-dependent decrease in adipocyte differentiation and triglyceride accumulation. mRNA expression of adipogenic transcription factors, such as peroxisome proliferator-activated receptor gamma, CCAAT/enhancer binding protein<em> ¦Á</em>, sterol regulatory element-binding protein 1 were decreased. The level of gene expression of adipogenesis and lipogenesis-related genes, such as adipocyte protein 2, lipoprotein lipase, acetyl-CoA carboxylase, ATP-citrate lyase and fatty acid synthase were decreased. These results suggest that AM-Ex alleviated risk factors related to obesity by modulating multiple pathways associated with adipogenesis.
%K Obesity
%K Adipocyte
%K Adipogenesis
%K Lipogenesis
%K Transcription Factor
%K Adipocyte Protein 2
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=90408