%0 Journal Article
%T Immunomodulatory potential of nanocurcumin-based formulation
%A Mahendra Kumar Trivedi
%A Sambhu Charan Mondal
%A Mayank Gangwar
%A Snehasis Jana
%J Inflammopharmacology
%P 609-619
%@ 1568-5608
%D 2017
%R 10.1007/s10787-017-0395-3
%X Vitamins, minerals, and nanocurcumin play a substantial role in various nutraceutical/pharmaceutical formulations that are widely used in therapeutics, cosmetics, and dietary supplements. The current study aimed to investigate the comparative in vitro immunomodulatory effect of a novel nanocurcumin-based formulation with curcumin in LPS-induced cytokine expression, NK cells¡¯ activity, and phagocytosis. The proinflammatory cytokines (TNF-¦Á, IL-1¦Â, and MIP-1¦Á) and NK cells¡¯ activity were measured in cell supernatants using ELISA assay; however, phagocytosis activity was performed using colorimetric analysis. The chemical characterization of novel nanocurcumin-based formulation using LC¨CMS (R t 19.02 min) and mass spectra analysis (m/z 369.04) confirmed the presence of the curcumin in highest peak concentration. MTT assay in three tested cell-lines showed that the formulation was found non-toxic at all the tested concentrations. The expression of TNF-¦Á, IL-1¦Â, and MIP-1¦Á in splenocytes was significantly (p ¡Ü 0.001) inhibited. Besides, the NK cells¡¯ activity and phagocytosis (macrophage) were increased significantly (p ¡Ü 0.001). Overall, the promising results of this study indicated the significant immunomodulatory effect of nanocurcumin-based formulation compared to the curcumin, which could be used against various inflammatory disorders such as allergy, asthma, autoimmune diseases, coeliac disease, inflammatory bowel disease, etc.
%K Nanocurcumin
%K Immunomodulation
%K Pro-inflammatory cytokines
%K Natural killer cells
%K Phagocytosis
%K Liquid chromatography-mass spectrometry
%U link.springer.com/article/10.1007%2Fs10787-017-0395-3