%0 Journal Article
%T Mitophagy in the Skeletal Muscle Is Suppressed in Spleen Qi Deficiency
%A Dan Ma
%A Wenjun Liu
%A Lu Wang
%A Xinzhu Xu
%A Lingzhi Wang
%A Huaxin Yu
%A Xudong Liu
%A Huihui Liu
%A Deshan Wang
%A Dehong Shan
%J Chinese Medicine
%P 11-18
%@ 2151-1926
%D 2019
%I Scientific Research Publishing
%R 10.4236/cm.2019.101002
%X <p style=\"text-align:justify;\">
	<b><span style=\"font-family:Verdana;\">Objective:</span></b><span style=\"font-family:Verdana;\"> Mitochondrial impairment in the skeletal muscle contributes to useless
of limbs in spleen qi deficiency</span><span style=\"font-family:Verdana;\">;</span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> however the genesis of such impairment is not clear. Herein,
PTEN-induced putative kinase 1 (PINK1)-Parkin pathway and mitophagy were
studied to explore the machinery of mitochondrial impairment. </span><b><span style=\"font-family:Verdana;\">Methods:</span></b><span style=\"font-family:Verdana;\"> 16 male SD rats were randomly divided in the control group and spleen qi
deficiency group (model group); transmission electron microscope was used to
observe mitochondrial morphology; mitochondrial oxidative phosphorylation was
assessed by testing mitochondrial membrane potential (MMP) and levels of ATP
and ROS; western blot was used to analyze expressions of PINK1, Parkin, microtubule-associated
protein 1 light chain 3-II (LC3-II) and p62. </span><b><span style=\"font-family:Verdana;\">Results:</span></b><span style=\"font-family:Verdana;\"> Compared with
those in the control group, mitochondria became small, less and scattered</span></span><span style=\"font-family:Verdana;\">, </span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\">MMP and the ATP level were
reduced, the ROS level was elevated, PINK1 expression was decreased, p62
expression was increased, but Parkin and LC3-II expressions were not altered,
in the model group. </span><b><span style=\"font-family:Verdana;\">Conclusions:</span></b><span style=\"font-family:Verdana;\"> Suppression of mitophagy might be
related to the mitochondrial damage in the skeletal muscle when spleen qi
deficiency develops.</span></span>
</p>
%K Spleen Qi Deficiency
%K Mitophagy
%K PINK1
%K Parkin
%K LC3
%K p62
%K Muscle
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=90290