%0 Journal Article
%T RNA Silencing-Mediated Control of <i>Odontoglossum ringspot virus</i> (ORSV) Infection
%A Xian Zhang
%A Yihua Hu
%A Zhijuan Chen
%A Pengcheng Zhang
%A Hongmei Li
%A Nongnong Shi
%J American Journal of Plant Sciences
%P 147-161
%@ 2158-2750
%D 2019
%I Scientific Research Publishing
%R 10.4236/ajps.2019.101013
%X <span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">&lt;i&gt;</span></i></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">Odontoglossum ringspot virus</span></i></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">&lt;/i&gt;</span></i></span></span><span><span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\"> (ORSV)
infects perennial orchids </span><i><span style=\"font-family:Verdana;\">(</span></i></span></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">&lt;i&gt;</span></i></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">Phalaenopsis amabilis</span></i></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">&lt;/i&gt;</span></i></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\">) and
causes a widespread viral disease. RNA-silencing of viral genes is a promising
and effective way of controlling viral infection in plants. An inverted repeat
(IR) fragment of the ORSV coat protein gene, </span></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\">&lt;i&gt;</span></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">cp</span></i></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">&lt;/i&gt;</span></i></span></span><span><span><span style=\"font-family:&quot;\"><span style=\"font-family:Verdana;\">,</span><i> </i><span style=\"font-family:Verdana;\">was inserted into the pXGY1 vector to
generate the silencing construct, pXGY1-ORSV, which was introduced into </span></span></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\">&lt;i&gt;</span></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">Nicotiana benthamiana</span></i></span></span><span style=\"font-family:Verdana;\"><span style=\"font-family:Verdana;\"><i><span style=\"font-family:Verdana;\">&lt;/i&gt;</span></i></span></span><span
%K ORSV
%K RNA Silencing
%K Agroinfiltration
%K Transient Expression
%K Transgenic Plant
%K Molecular Analysis
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=90024