%0 Journal Article
%T Tetra Primer ARMS PCR Optimization to Detect Single Nucleotide Polymorphism of the <i>KLF</i>14 Gene
%A Ana Sofia Zabala
%A Miriam Ester Vasquez Gomez
%A Micaela Fernanda Alvarez
%A Susana Siewert
%J Open Access Library Journal
%V 4
%N 12
%P 1-14
%@ 2333-9721
%D 2017
%I Open Access Library
%R 10.4236/oalib.1104145
%X <div style="text-align:justify;">
	<span style="line-height:1.5;">GWAS (genome-wide association studies) have associated Type 2 Diabetes Mellitus with the single nucleotide polymorphism (SNPs) rs972283 (A/G) of KLF14 gene with in a global population. The most common techniques used to analyze SNPs are time consuming, multi-step process and require expensive instruments. Therefore, in order to overcome these problems, we have developed a new, rapid and cost effective T-ARMS PCR assay to genotype rs972283. However, the optimization step can be hardworking and laborious. Hence, we propose to demonstrate and discuss critical steps for its development, in a way to provide useful information. In a first step, we design and validate two specific primer pair for T-ARMS PCR. Later, the amplification conditions were optimized for DNA concentration, annealing temperature, Taq DNA polymerase units and primers concentration. The last one was considered the main interference factor for a correct amplification and appropriate band intensity. Finally, the results obtained by T-ARMS PCR were concordant with sequencing. T-ARMS PCR assay developed in our laboratory for genotyping rs972283 (A/G) of KLF14 gene is time saving and cost-effective compared to the available methods used for SNP studies.</span> 
</div>
%K rs972283 (A/G)
%K KLF14 Gene
%K Type 2 Diabetes Mellitus
%U http://www.oalib.com/paper/5291500