%0 Journal Article
%T Determining a Diagnostic Cut-Off on Fluorescence Polarization Assay (FPA) for Bovine Brucellosis in Carchi, Ecuador
%A Ibarra Marcelo
%A Benavides Hern¨¢n
%A Salgado Ruth
%A Guti¨¦rrez Milena
%A Garc¨ªa Judith
%A Pe£¿a Julio
%A Herrera David
%A Mina Jorge
%A Campos Mart¨ªn
%A Puga Byron
%J Open Journal of Animal Sciences
%P 425-432
%@ 2161-7627
%D 2017
%I Scientific Research Publishing
%R 10.4236/ojas.2017.74033
%X Serology is the foundation
of any brucellosis control and eradication program worldwide, thus it is
important to define accuracy diagnostics assays and cut-off of those assays, due to variations from country
to country and even among specific areas in the country. The variation of
cutoff values depended on: prevalence of disease, vaccination status, animal
management, and control and eradication programs. Therefore, a cut-off for the diagnosis of bovine
brucellosis through fluorescence polarization assay (FPA) in Carchi¡ªEcuador was
determined. The survey has been carried out in Carchi province of Ecuador, who
is considered a province of high prevalence of brucellosis and the vaccination
status is unknown due to the lack of
registers. Sera samples (n = 200)
were obtained from individual cows from randomly selected herds. Blood sera
were tested through Fluoresce Polarization Assay (FPA) and competitive
enzyme-linked inmunosorbent assay (cELISA) as confirmatory test, and then receiver operating characteristic (ROC)
analysis was done. The sensitivity and specificity values of FPA were 88.7% and 92.50% respectively using a cut-off of 89.90 mP. Moreover, the area under the curve showed
that 92.2% is the probability accuracy of the test. The advantage of the FPA is that it is a test with good
%K Brucella
%K Diagnosis
%K Fluorescence Polarization
%K Sensitivity
%K Specificity
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=79517