%0 Journal Article
%T ADSL, AMPD1, and ATIC Expression Levels in Muscle and Their Correlations with Muscle Inosine Monophosphate Content in Dapulian and Hybridized Pig Species
%A Rongsheng Zhu
%A Yanping Wang
%A Huaizhong Wang
%A Song Lin
%A Shouli Sun
%A Baohua Huang
%A Hongmei Hu
%J Open Journal of Animal Sciences
%P 393-404
%@ 2161-7627
%D 2017
%I Scientific Research Publishing
%R 10.4236/ojas.2017.74030
%X We investigated the relationship between muscle
inosine monophosphate (IMP) content and mRNA levels of ADSL, AMPD1, and ATIC in
Dapulian (DPL), Landrace กม Dapulian (LDPL), and Duroc กม Landrace กม Dapulian
(DLDPL) hybridized pigs. Methods:<span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">The total RNA in longissimus dorsi </span><span style=\"font-family:Verdana;\">was</span><span style=\"font-family:Verdana;\"> isolated from Dapulian (DPL), Landrace กม Dapulian (LDPL) and Duroc กม
Landrace กม Dapulian (DLDPL) hybridized pigs, weighed about 95.0 kg, n =
8/species. The internal genes with highest stability (YWHAZ and RPL4) were
chosen from 11 common internal genes using Quantitative real-time PCR (qPCR)
and geNorm software. The mRNA level</span><span style=\"font-family:Verdana;\">s</span><span style=\"font-family:Verdana;\"> of ADSL, AMPD1 and ATIC genes were corrected with YWHAZ and RPL4 genes.
The muscular IMP content was determined by HPLC.</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">The muscular IMP content in DPL was higher than that in LDPL and DLDPL,
25.00% (p</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">&lt;</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">0.05) and 15.56% (p</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">&gt;</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">0.05), respectively. The muscular mRNA level of ADSL gene in DPL and LDPL </span><span style=\"font-family:Verdana;\">was</span><span style=\"font-family:Verdana;\"> higher than that in DLDPL, 24.14% and 12.07%, respectively (p</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">&lt;</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">0.05). The muscular mRNA level of ATIC gene in DPL and LDPL </span><span style=\"font-family:Verdana;\">was</span><span style=\"font-family:Verdana;\"> higher than that in DLDPL, 66.67% and 33.33%, respectively (p</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">&lt;</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">0.05). The muscular mRNA level of AMPD1 gene in DPL and LDPL </span><span style=\"font-family:Verdana;\">was</span><span style=\"font-family:Verdana;\"> higher than that in DLDPL, 14.49% and 33.26%, respectively. Furthermore,
the IMP content was
%K Pigs
%K ADSL Gene
%K AMPD1 Gene
%K ATIC Gene
%K Correlation
%K Inosine Monophosphate
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=79068