%0 Journal Article
%T A New Vaccine Strategy of Dendritic Cell Presented Kinetoplastid Membrane (KMP-11) as Immunogen for Control against Experimental Visceral Leishmaniasis
%A Rajesh Chaudhary
%A Ajay Amit
%A Akhilesh Kumar
%A Manas R. Dikhit
%A Krishna Pandey
%A Pradeep Das
%A Sanjiva Bimal
%J Modern Research in Inflammation
%P 15-28
%@ 2169-9690
%D 2017
%I Scientific Research Publishing
%R 10.4236/mri.2017.63003
%X Available reports suggest that, <i>Leishmania donovani </i>antigen KMP-11 may
be significant in the modulation of immune responses in visceral leishmaniasis (VL).
This study evaluated vaccine prospect of presentation of KMP-11 antigen through
murine dendritic cells against VL in infected BALB/c mice. We report here that immunization
with KMP-11 delivered through bone marrow derived dendritic cells can lead to killing
of <i>L. donovani</i> in infected BALB/c mice.
Furthermore, the strategy to use KMP-11 as vaccine delivered through DCs can stimulate
the production of IFN-g, IL-12, IL-2R and TNF-¦Á with
concomitant down-regulation of IL-10 and IL-4. Furthermore, anti-leishmanial defence function (ROS) of splenocytes was observed increased in the presence
of DC-delivered KMP-11 vaccination accompanied with an increased p38-MAPK signalling
in vaccinated splenocytes. We summarized from our data that
KMP-11 delivered through DCs has potential for eliciting protective immunity through
pro-inflammatory cytokines (IFN-¦Ã, IL-12, IL-2, TNF-¦Á) following an up-regulation
in signalling event of p38-MAPK. Therefore the study suggests a new control strategy
against VL in future.
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%K Visceral Leishmaniasis
%K Kinetoplastid Membrane Protein 11
%K Soluble Leishmania Antigen
%K Interferon-&lt
%K sub&gt
%K &amp
%K upsih
%K &lt
%K /sub&gt
%K Interleukin-12
%K Interleukin-10
%K Dendritic Cell Primed KMP-11
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=78619