%0 Journal Article
%T Vitamin D Receptor Expression in Lumbar Disc Degeneration Patients
%A Mahmoud M. Taha
%A Norhan A. Sabbah
%A Noha A. Rezk
%A Hosam Mansour
%J Open Journal of Modern Neurosurgery
%P 19-33
%@ 2163-0585
%D 2017
%I Scientific Research Publishing
%R 10.4236/ojmn.2017.72003
%X Background:<span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">Lumbar disc degeneration [LDD] is the main cause of low back pain, and is
a medical circumstance that poses a great load on the global health care system
with serious socioeconomic consequences. The aim of this study was to determine
the association of vitamin D receptors (VDR) gene Fok1 polymorphism, VDR
expression and vitamin D level with LDD susceptibility and severity in
Egyptians.</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">Materials and methods: </span><span style=\"font-family:Verdana;\">A total of unrelated 169 female patients with low back pain diagnosed as
having lumbar disc degeneration and 169 controls were enrolled in the study.
VDR Fok1 polymorphism was determined using restriction fragment length
polymorphism</span><span style=\"font-family:Verdana;\">-</span><span style=\"font-family:Verdana;\">polymerase chain reaction [PCR-RFLP]. VDR expression was measured by
reverse transcriptase PCR, while, serum levels of vitamin D w</span><span style=\"font-family:Verdana;\">ere</span><span style=\"font-family:Verdana;\"> measured by high performance liquid chromatography [HPLC].</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">Results:</span><i><span style=\"font-family:;\" \"=\"\"> </span></i><span style=\"font-family:Verdana;\">Subjects with VDR Fok1 ff genotype were significantly more likely to
develop</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">LDD in Egyptians and associated with multilevel disc degeneration and
disc herniation</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">(adjusted OR</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">=</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">7.9, 95% CI</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">= 2.3</span>-<span style=\"font-family:;\" \"=\"\"><span style=\"font-family:Verdana;\">26.6 and</span><i><span style=\"font-family:Verdana;\"> P</span></i><span style=\"font-family:Verdana;\"> = 0.01) and (adjusted OR</span></span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">=</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">2.2,
95% CI</span><span style=\"font-family:;\" \"=\"\"> </span><span style=\"font-family:Verdana;\">= 1.6</span>-<span
%K Lumbar Disc
%K Vitamin D
%K Degeneration
%K Polymorphism
%K PCR
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=75394