Background: Recently the data has been increasing on the association between Helicobacter pylori infection and thrombocytopenia characterization specifically in developing countries. Objective: This study was aimed to evaluate platelet count and platelet indices in Helicobacter pylori infected patients. Materials and Method: This is a case control study conducted in the Faculty of Medical Laboratory at Alneelain University, Khartoum-Sudan. A total of 240 participants was enrolled in this study; 120 of them are a patient group known diagnosed with H. pylori infection; 60 (50%) male, 60 (50%) female; their mean age is (30.22 ¡À 1.92) years designed as patient group. And another 120 designated as normal, healthy control group; 60 (50%) male and 60 (50%) female. Blood samples were collected from each subject in ethylene diamine tetra acetic acid (EDTA). The platelet count and platelet Indices were done by using full automated hematological Analyzer Sysmex (KXN-21) Japan. The data were analysed by using (SPSS) version 20. Result: Platelet count and platelet distribution width (PDW) were shown statistically significantly lower; on the other hand the MPV was found higher in patients with H. pylori infection compared with normal healthy control group with p value 0.03, 0.01 and 0.00 respectively. Conclusion: Mild thrombocytopenia with elevated in mean platelet volume (MPV) and low on the platelet distribution width (PDW) were found in patients with H. pylori infection.