%0 Journal Article
%T OSW-1 Induced Apoptosis in Hepatocellular Carcinoma through Generation of ROS, Cytochrome C and Noxa Activation Independent of p53 with Non-Activation of Caspase-3
%A Xiaochen Liu
%A Jingchao Liang
%A Jichun Jin
%A Haiyang Li
%A Bosheng Mei
%A Xinglin Jin
%J Chinese Medicine
%P 1-9
%@ 2151-1926
%D 2017
%I Scientific Research Publishing
%R 10.4236/cm.2017.81001
%X Aim: To study the antitumor mechanism of OSW-1 in hepatocellular
carci<span style=\"font-family:Verdana;\">noma. </span><span style=\"font-family:Verdana;\">Materials and Methods: The expression profiling microarray was</span><span> </span><span style=\"font-family:Verdana;\">carried
out to extract RNA from SK-Hep-1 which suffered from OSW-1</span><span style=\"font-family:Verdana;\">.</span> <i><span style=\"font-family:Verdana;\">¦Ñ</span></i><sup><span style=\"font-family:Verdana;\">0</span></sup><span style=\"font-family:Verdana;\">-SK-Hep-1 was maintained SK-Hep-1 in MEM
containing 100 ¦Ìg/L ethi</span><span style=\"font-family:Verdana;\">dium bromide (EB), 1 mM sodium pyruvate and 50
¦Ìg/ml uridine for 40 days. </span><span style=\"font-family:Verdana;\">Then confirmed
COX-I and COX-II of mitochondrial DNA were knocked</span><span style=\"font-family:Verdana;\"> out. Cells suffered
from OSW-1 or doxorubicin. Then cells were washed twice </span><span style=\"font-family:Verdana;\">with cold PBS and incubated with DCFH-DA. Fluorescent signal was record</span><span style=\"font-family:Verdana;\">ed </span><span style=\"font-family:Verdana;\">by using Infinite 200 Pro multimode Plate
readers. Results: OSW-1</span><span style=\"font-family:Verdana;\"> elevates generation of ROS and Cytochrome C
which are associated with the induction of apoptosis in SK-Hep-1 cells. We also
demonstrate that OSW-1 </span><span style=\"font-family:Verdana;\">does not depend on p53
to up</span><span style=\"font-family:Verdana;\">-regulate the BH3-only protein Noxa. What is more noteworthy that
the Caspase-9 and FADD are down-regulated in above </span><span style=\"font-family:Verdana;\">process. Conclusion: OSW-1 induced special apoptosis is different from
the</span><span style=\"font-family:Verdana;\"> mitochondrial death pathway and the death receptor pathway and final
result </span><span style=\"font-family:Verdana;\">is not Caspase family¡¯s activating.
This provides a novel theory that nonma</span><span style=\"font-family:Verdana;\">lignant cells are significantly
less sensitive to OSW-1 than cancer cell lines.</span>
%K OSW-1
%K Hepatocellular Carcinomas
%K ROS
%K Cytochrome C
%K Mitochondrial Death Pathway
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=74699