%0 Journal Article %T Detection and Characterization of <i>¦Ā</i>-Lactamase Encoding Genes in Carbapenem Non-Susceptible Gram-Negative Bacteria and Susceptibility of Isolates to Ceftazidime-Avibactam at a New York City Community Hospital %A Carl Urban %A Rita Colon-Urban %A Vincent J. LaBombardi %A Noriel Mariano %A Nishant Prasad %A Geeti Dhillon %A Marina Guralnik %A Sorana Segal-Maurer %J Open Journal of Medical Microbiology %P 150-157 %@ 2165-3380 %D 2016 %I Scientific Research Publishing %R 10.4236/ojmm.2016.64020 %X A surveillance study was undertaken to identify prominent ¦Ā-lactamase encoding genes in 131 carbapenem non-susceptible gram-negative clinical isolates at a New York City community hospital. KPC carbapenemases were detected in 89% of Enterobacteriaceae as well as additional TEM, SHV, and CTX-M class A enzymes. OXA-23 and OXA-24 were the prevalent class D carbapenemases identified in Acinetobacter species. One OXA-23 in M. morganii and one OXA-48 in K. pneumoniae were also identified. Among class C ¦Ā-lactamases CMY, ACT/MIR, DHA, and FOX were detected. The in vitro activity of ceftazidime-avibactam by E-test methodology was tested with minimal inhibitory concentrations (MIC) of ”Ü3 ¦Ģg/ml for 97.8% of all Enterobacteriaceae, MIC_50/90 of 16/>256 ¦Ģg/ml for carbapenem non-susceptible Acinetobacter, and 3/6 ¦Ģg/ml for carbapenem non-susceptible Pseudomonas aeruginosa. Periodic surveillance of isolates to characterize current and emerging ¦Ā-lactamase genotypes present in local isolates may help identify outbreak situations, provide assistance to infection control and antibiotic stewardship programs, and potentially improve patient outcomes. %K Carbapenem Non-Susceptible %K Check-MDR CT103 XL Microarray %K < %K i> %K ¦Ā< %K /i> %K -Lactamase Detection %K Resistance Mechanisms %K Ceftazidime-Avibactam %U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=72843