%0 Journal Article
%T Clinical Isolates of <i>Staphylococcus aureus</i> Show Variation in <i>¦Â</i>-Lactamase Production and Are More Susceptible to Antibiotics Conjugated with <i>¦Â</i>-Lactamase Inhibitors
%A Uzal Umar
%A Umar Ahmed Faruk
%A Damoroem M. Tanko
%A Mohammed B. Yerima
%J Open Journal of Medical Microbiology
%P 143-149
%@ 2165-3380
%D 2016
%I Scientific Research Publishing
%R 10.4236/ojmm.2016.64019
%X <i><span style=\"font-family:&quot;\">¦Â</span></i><span style=\"font-family:&quot;\">-Lactam antibiotics are a
cornerstone in the treatment of bacterial infections on account of its high
therapeutic index and selective toxicity¡ªthey act by inhibiting the
biosynthesis of peptidoglycan</span><span style=\"font-family:&quot;\">,</span><span style=\"font-family:&quot;\"> a key
component in bacterial cell wall. Ninety (90) clinical specimens obtained from
the microbiology unit Specialist Hospital Bauchi were screened for <i>S.</i></span><i><span style=\"font-family:&quot;\"> </span></i><i><span style=\"font-family:&quot;\">aureus</span></i><span style=\"font-family:&quot;\">, positive isolates were
examined for <i>¦Â</i>-Lactamase expression </span><span style=\"font-family:&quot;\">by </span><span style=\"font-family:&quot;\">using two Penicillin G
concentrations (5000 IU/ml and 25</span><span style=\"font-family:&quot;\">,</span><span style=\"font-family:&quot;\">000 IU/ml) in acidometric agar technique with phenol red as indicator, </span><span style=\"font-family:&quot;\">and </span><span style=\"font-family:&quot;\">the susceptibility pattern
of the isolates to <i>¦Â</i>-Lactam
antibiotics was also determined. <i>S.</i></span><i><span style=\"font-family:&quot;\"> </span></i><i><span style=\"font-family:&quot;\">aureus</span></i><span style=\"font-family:&quot;\"> prevalence of 31% (28/90)
was obtained</span><span style=\"font-family:&quot;\">,</span><span style=\"font-family:&quot;\"> of
which 96% (27/28) of strains were <i>¦Â</i>-Lactamase
positive in the standard test, while 63% (17/27) were able to hydrolyze
penicillin G concentration of 25</span><span style=\"font-family:&quot;\">,</span><span style=\"font-family:&quot;\">000</span><span style=\"font-family:&quot;\"> </span><span style=\"font-family:&quot;\">IU/ml
(5X the concentration in the standard test), </span><span style=\"font-family:&quot;\">and </span><span style=\"font-family:&quot;\">a strain was found to be <i>¦Â</i>-Lactamase negative. The resistance to five <i>¦Â</i></span><span style=\"font-family:&quot;\">-</span><span style=\"font-family:&quot;\">Lactams</span><span style=\"font-family:&quot;\">,</span><span style=\"font-family:&quot;\"> ampicillin, cephalexin,
amoxicillin, cloxacillin and flucloxaillin</span><span style=\"font-family:&quot;\">,</span><span style=\"font-family:&quot;\"> were 100</span><span style=\"font-family:&quot;\">%</span><span style=\"font-family:&quot;\">, 96</span><span style=\"font-family:&quot;\">%</span><span style=\"font-family:&quot;\">, 89</span><span
%K &lt
%K i&gt
%K ¦Â&lt
%K /i&gt
%K -Lactamase
%K Peptidoglycan
%K Transpeptidation
%K Haemolysis
%K Resistance
%K Antibiotics
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=72707