%0 Journal Article
%T Characterization of Phenotype and Genotype of Biofield Treated Enterobacter aerogenes
%A Mahendra Kumar Trivedi
%A Alice Branton
%A Dahryn Trivedi
%A Gopal Nayak
%A Mayank Gangwar and Snehasis Jana
%J Translational Medicine
%D 2015
%R 10.4172/2161-1025.1000155
%X Enterobacter aerogenes£¿(E. aerogenes) has been commonly described as a versatile opportunistic pathogen in hospital infections. The aim of the present work was to evaluate the impact of biofield treatment on£¿E. aerogenes£¿for its phenotypic and genotypic characteristics.£¿E. aerogenes£¿bearing ATCC 13048 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to Mr. Trivedi¡¯s biofield treatment and analyzed for antimicrobial susceptibility, minimum inhibitory concentration (MIC), biochemical reactions, and biotype using automated MicroScan Walk-Away£¿system. In addition, treated group of£¿E. aerogenes£¿was evaluated for DNA polymorphism by Random Amplified Polymorphic DNA (RAPD) and 16S rDNA sequencing to establish the phylogenetic relationship of£¿E. aerogenes£¿with different closely related bacterial species. Antimicrobial susceptibility results showed an alteration of 14.28% among twenty-eight tested antimicrobials. Similarly, 15.65% tested antimicrobials showed an alteration in MIC values. Chloramphenicol showed improved sensitivity i.e. resistant to susceptible after biofield treatment, with the support of decreased MIC by two folds (i.e. >16 to ¡Ü8 ¦Ìg/mL). Norfloxacin also showed decrease MIC by two folds (i.e. 8 to ¡Ü4 ¦Ìg/mL) as compared to control. Biofield treatment showed an impact on biochemical reactions (9.09%) followed by a change in biotype number (7770 5272) in treated group with respect to control (7770 5372). Using RAPD analysis, sample showed an average range of 4 to 42% of polymorphism, while 16S rDNA study showed that treated sample was detected as Kluyvera cryocrescens (GenBank Accession Number: AM184245) with 97% identity of gene sequencing data, which was nearest homolog species to£¿Enterobacter aerogenes£¿strain: C1111 (Accession No. AB244467). These results suggest that Mr. Trivedi¡¯s unique biofield treatment can alter the antimicrobial sensitivity pattern, thus it can be used as alternate energy medicine in future.
%K Enterobacter aerogenes
%K Biofield treatment
%K Phenotyping
%K Polymorphism
%K RAPD
%K 16S rDNA analysis
%U http://www.omicsonline.org/open-access/characterization-of-phenotype-and-genotype-of-biofield-treated-enterobacter-aerogenes-2161-1025-1000155.pdf