%0 Journal Article
%T Correlation between Sequencing Results from Liquid Plasma and Dried Plasma Spot (DPS) for Determination of HIV Type 1 Non-B Subtypes
%A Erick Ntambwe Kamangu
%A Adawaye Chatt¨¦
%A Dolores Vaira
%A Patrick de Mol
%A Georges Lelo Mvumbi
%A Richard Lunganza Kalala
%A Marie-Pierre Hayette
%J Open Access Library Journal
%V 4
%N 2
%P 1-7
%@ 2333-9721
%D 2017
%I Open Access Library
%R 10.4236/oalib.1102922
%X <div style="text-align:justify;">
	<span style="line-height:1.5;">Background:
The blotting paper is an alternative to the collection of blood in the tubes
for analysis, especially in the field of Human Immunodeficiency Virus
infection. This technique allows to easily send the collected samples to specialized
laboratories while limiting the stresses of storage and transport. Objective:
The objective of this study was to compare the results of sequencing performed
on liquid plasma and Dried Plasma Spot (DPS) for the variants of HIV-1 non-B. Methodology:
Fifty subjects diagnosed positive for HIV Type 1 using the Rapid Screening
Tests voluntarily participated in this study. Two hundred microlite</span><span style="line-height:1.5;">rs of plasma
are deposited on blotting paper Whatman 903 and 500 ¦Ìl in a
micro tube. RNA was extracted from 140 ¦Ìl of
plasma fluid and from a piece of DPS of 5 mm of
diameter using the QIAamp RNA Mini Kit QIAGEN<img src="http://www.oalib.com:80/image/DEF_PAPERATTACHED_PATH/20170204150500_77.bmp" alt="" /></span><span style="line-height:1.5;">. After extraction, the Viral Load (VL) was performed on each sample of
liquid plasma. A Reverse Transcription PCR and Nested PCR were used to amplify
the regions of interest on the Protease and Reverse Transcriptase for
subsequent sequencing. Results: Protease and Reverse Transcriptase were
amplified and sequenced respectively for 44 (88%) and 48 (96%) with the liquid
plasma samples and 40 (80%) and 45 (90%) with the DPS. The results of Viral
Loads were in the range of 2.5 log</span><sub>10</sub><span style="line-height:1.5;"> and 6.5 log</span><sub>10</sub><span style="line-height:1.5;">. The
results of sequencing are comparable for plasma samples and DPS. The
correlation coefficient (R</span><sup>2</sup><span style="line-height:1.5;">) between the two methods is good (R</span><sup>2</sup><span style="line-height:1.5;"> = 0.903, p &lt; 0.001). Conclusion: Liquid Plasma and Dried Plasma Spot give highly
correlated results for sequencing strains of HIV type 1 non-B.</span>
</div>
%K Sequencing
%K Liquid Plasma
%K Dried Plasma Spot
%K HIV-1 Non-B
%K Kinshasa
%U http://www.oalib.com/paper/5271394