%0 Journal Article
%T Role of <i>¦Á</i>-Tubulin Acetylation and Protein Kinase D2 Ser/Tyr Phosphorylation in Modulation by Ghrelin of <i>Porphyromonas gingivalis</i>-Induced Enhancement in Matrix Metalloproteinase-9 (MMP-9) Secretion by Salivary Gland Cells
%A Bronislaw L. Slomiany
%A Amalia Slomiany
%J Journal of Biosciences and Medicines
%P 82-94
%@ 2327-509X
%D 2016
%I Scientific Research Publishing
%R 10.4236/jbm.2016.47009
%X Matrix
metalloproteinas-9 (MMP-9) is a glycosylated endopeptidase, and hence its
processing between the endoplasmic reticulum (ER), Golgi and trans-Golgi (TGN)
network remains under a strict control of factors that affect the microtubule
(MT) stabilization, and the recruitment and activation of coat and cargo
proteins, including ADP-ribosylation factors (Arfs) and protein kinase D (PKD).
Here, we report on the factors implicated in the regulation of MMP-9 secretion
by salivary gland acinar cells in response to <i>P. gingivalis </i>LPS, and the effect of hormone, ghrelin. We show that
the LPS-elicited induction in MMP-9 secretion is associated with the increase
in <i>¦Á</i>-tubulin acetylation and the
enhancement in MT stabilization, while the modulatory effect of ghrelin is
reflected in a decrease in <i>¦Á</i>-tubulin
acetylation. Further, the effect of the LPS occurs in concert with up-regulation
in Arf-guanine nucleotide exchange factor (GEF)-mediated Arf1 activation and
the TGN recruitment of PKD2, while ghrelin exerts the modulatory effect on
Arf-GEF activation. Moreover, we reveal that the LPS-induced up-regulation in
MMP-9 secretion is reflected in a marked increase in PKC<i>¦Ä</i>-mediated PKD2 phosphorylation on Ser, while the modulatory effect
of ghrelin is manifested by the SFK-PTKs-dependent phosphorylation of PKD2 on
Tyr. The findings demonstrate that MT stabilization along with Arf-GEF-mediated
Arf1/PKD2 activation play a major role in <i>P.
gingivalis </i>LPS-induced up-regulation in salivary gland acinar cell MMP-9
secretion, and point the modulatory mode of action by ghrelin.
%K &lt
%K i&gt
%K Porphyromonas gingivalis&lt
%K /i&gt
%K Oral Mucosa
%K Ghrelin
%K MMP-9
%K &lt
%K i&gt
%K ¦Á&lt
%K /i&gt
%K -Tubulin Acetylation
%K Arf1
%K PKD2 Ser/Tyr Phosphorylation
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=68146