We evaluated centrally acting analgesic potential using tail immersion test and peripheral pharmacological actions using acetic acid induced writhing test in rats. The crude extracts of Trema guineensis leave plant were found to have significant analgesic activity at the intraperitoneal dose of 200 mg/kg body weight in two models. In the tail immersion test Trema guineensis increased significantly (P < 0.001) in comparison to the control group (NaCl). The ethanolic (7.57 ¡À 0.14 s) and aqueous (8.88 ¡À 0.12 s) extracts reached their maximum in reaction time successively at 30and 45 min. The aim of this study is also to determine the trace elements content in suffering rats of pain induced by acetic acid, using Atomic Spectrophotometer Absorption. Our results showed that trace element concentrations in Trema guineensis ethanolic extract in rats were as follows: Zn (6.92 ¡À 0.39 mg/L), Fe (37.95 ¡À 0.04 ¦Ìmol/L), Cu (8.16 ¡À 0.12 mg/L) and Mn (0.56 ¡À 0.12 mg/L). We found following values with aqueous extract compared to NaCl as control: Zn (5.03 ¡À 0.47 mg/L), Fe (31.08 ¡À 0.07 ¦Ìmol/L), Cu (6.69 ¡À 0.53 mg/L) and Mn (0.51 ¡À 0.07 mg/L). The intake of trace elements by TGE would lessen the pain generated in acetic acid in rats; this action is much more remarkable with the ethanol extract.