%0 Journal Article
%T Adiponectin Supports Human Glioma Cells Survival against Temozolomide through Enhancement of Autophagic Response in Glioma Cells
%A Peng Sun
%A Feng Yan
%A Jinning Song
%A Xudong Ma
%J Journal of Biosciences and Medicines
%P 1-9
%@ 2327-509X
%D 2016
%I Scientific Research Publishing
%R 10.4236/jbm.2016.44001
%X Objective: To investigate the role of adiponectin in human glioma cell lines against the temozolomide and the molecular regulation mechanism. Methods: Human glioma cell lines U251 and U-87MG were cultured in Dulbecco¡¯s modified eagle medium (DMEM) containing 4500 mg/L glucose. MTT was used to measure cell growth ratio. Western blot was used to detect the protein levels of autophagy-related protein (Beclin 1, LC3 I/II, p62) and phosphorylated AMPK (p-AMPK) in human glioma cell lines. After AICAR and Compound C were administered, the change of p-AMPK and the autophagy level were examined by western blot. Results: While adiponectin stimulates AMPK in phosphatase and up-regulates the level of autophagy, human glioma cell lines obtain more resistance against the temozolomide, which is facilitated by AICAR and weakened by Compound C. Conclusion: As an important adipokine, adiponectin can up-regulate the glioma cell autophagy by activating the AMPK signaling pathway which increases the resistance of glioma cells to temozolomide.
%K Human Glioma Cell Lines
%K Adiponectin
%K Autophagy
%K Temozolomide
%K AMPK
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=64918