%0 Journal Article
%T Effect of Electro-Acupuncture on Expression of Circadian Clock Gene Per2 and Bmal1 in Sleep Deprivation Rat
%A Jie Yu
%A Jia Chen
%A Tianjun Hu
%A Shanghan Guo
%A Xiulian Wang
%A Yinyi Guo
%A Xiaoyin Chen
%A Canghuan Zhao
%J Chinese Medicine
%P 16-24
%@ 2151-1926
%D 2016
%I Scientific Research Publishing
%R 10.4236/cm.2016.71003
%X Objective: The objective is to observe the treatment effect of electro-acupuncture (EA) on core circadian clock gene Per2 and Bmal1 expression in hypothalamus of sleep-deprivation (SD) rats. Methods: Thirty-two Wistar male rats were randomly divided into 4 groups. Mice in the blank control group did not receive any treatment; the remaining groups were applied with para-chlorophenylalanine (PCPA) 300 mg/kg intraperitoneal injection for 2 days. Diazepam group received intraperitoneal injection of Diazepam (0.9 mg/kg, i.p.) one time a day for 5 days, while M group was treated with saline (0.9 mg/kg, i.p.) at the same time. Rats in EA group were given EA treatment, 20 minutes, once a day for 5 days, and rats in remaining groups were put into fixation-machine for the same time everyday, lasting for 5 days. Rats were sacrificed after anesthesia at the 8th day. Real-time PCR was adopted to detect the expression in clock gene Per2 and Bmal1 of each group. Results: Compared with blank control group, the expression of Per2 was significant decreased in PCPA model group (P < 0.05), the expression of Bmal1 was increased in PCPA model group (P < 0.05). Compared with PCPA model group, the expression of Per2 were significant enhanced in EA group and Diazepam group (P < 0.05). Simultaneously, compared with PCPA group, the expression of Bmal1 was no statistically significant in EA group and Diazepam groups (P > 0.05). Conclusion: EA can significant up-regulate the expression of Per2 in SD rats, and down-regulate gene Bmal1 expression, and benefiting the weight of rats. Thus, EA is a potentially promising intervention to treat sleep-deprivation.
%K Electro-Acupuncture
%K Sleep-Deprivation
%K Circadian Rhythm
%K Per2
%K Bmal1
%K Suprachiasmatic Nucleus (SCN)
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=64509