%0 Journal Article
%T Leader-Containing Uncapped Viral Transcript Activates RIG-I in Antiviral Stress Granules
%A Seong-Wook Oh&nbsp
%A Koji Onomoto&nbsp
%A Mai Wakimoto&nbsp
%A Kazuhide Onoguchi&nbsp
%A Fumiyoshi Ishidate&nbsp
%A Takahiro Fujiwara&nbsp
%A Mitsutoshi Yoneyama&nbsp
%A Hiroki Kato&nbsp
%A Takashi Fujita
%J PLOS Pathogens
%D 2016
%I Public Library of Science (PLoS)
%R 10.1371/journal.ppat.1005444
%X RIG-I triggers antiviral responses by recognizing viral RNA (vRNA) in the cytoplasm. However, the spatio-temporal dynamics of vRNA sensing and signal transduction remain elusive. We investigated the time course of events in cells infected with Newcastle disease virus (NDV), a non-segmented negative-strand RNA virus. RIG-I was recruited to viral replication complexes (vRC) and triggered minimal primary type I interferon (IFN) production. RIG-I subsequently localized to antiviral stress granules (avSG) induced after vRC formation. The inhibition of avSG attenuated secondary IFN production, suggesting avSG as a platform for efficient vRNA detection. avSG selectively captured positive-strand vRNA, and poly(A)+ RNA induced IFN production. Further investigations suggested that uncapped vRNA derived from read-through transcription was sensed by RIG-I in avSG. These results highlight how viral infections stimulate host stress responses, thereby selectively recruiting uncapped vRNA to avSG, in which RIG-I and other components cooperate in an efficient antiviral program.
%U http://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1005444