%0 Journal Article
%T endogenousneuregulin-1expressionintheanteriorpituitaryoffemalewistar-furthratsduringtheestrouscycle
%A 赵炜疆
%A 任颂光
%J 南方医科大学学报
%D 2011
%X abstract:objectivetostudythechangesinendogenousneuregulin-1(nrg1)expressionintheanteriorpituitaryoffemalewistar-furthratsindifferentphasesoftheestrouscycle.methodsfemalewistar-furthratsduringestrouscycleswereused.rt-pcrwasemployedtostudythechangesintheexpressionofnrg1isoformsandtheircognatereceptorserbb-2anderbb-4intheanteriorpituitaryindifferentphasesoftheestrouscycle.westernblottingwasusedtodetectnrg1expressionattheproteinlevel.immunofluorescencestainingwasusedtoidentifyhypophysealcellsexpressingnrg1andobservethelocalizationanddistributionofnrg1andfunctionalphosphorylationoferbb-4.theco-expressionofnrg1anderbb-4intheanteriorpituitaryofrhesusmonkeywasalsoinvestigated.resultssomeofthenrg1isoforms,especiallytypeiiinrg1s,wereexpressedatahigherlevelduringtheestrouscyclei(e1)andestrouscycleii(e2),aresultconsistentwiththatofwesternblottingforsamplesoftheanteriorpituitariescollectedatthesephases.immunofluorescencestainingidentifiedthegonadotrophsasthemainsourceofnrg1,andshowedanextensivedistributionofnrg1intheanteriorpituitaryine1ande2phasesaccompaniedbyapparentphosphorylatedactivationoferbb-4.adjacentdistributionofnrg1-anderbb-4-positivecellswasalsoobservedintheanteriorpituitaryofmalerhesusmonkeys.conclusionourresultsprovideevidencefortheexpressionofmultiplenrg1isoformsandthepresenceofnrg1/erbb-4signalingintheanteriorpituitaryoffemalewistar-furthrats.thissignalingdemonstratesanestrouscyclephase-relatedpattern.additionally,nrg1/erbb-4-basedjuxtacrinesignalingmayexistintheanteriorpituitaryofmalenon-humanprimate.
%K neuregulin-1
%K estrouscycle
%K anteriorpituitary
%K phospho-erbb-4
%K wistar-furthrats
%U http://www.j-smu.com/oa/darticle.aspx?type=view&id=201106921