%0 Journal Article
%T 石榴叶多酚的纯化及抗氧化活性研究
%A 邢佳
%A 陆文娟
%A 赵云霞
%A 陶明煊
%J 南京师范大学学报(自然科学版)
%D 2015
%X 采用大孔树脂对石榴叶粗多酚进行纯化，得到3种不同纯度的石榴叶多酚，对这3种多酚的抗氧化活性进行研究并与芦丁对比.结果显示，最佳纯化工艺条件为：上柱质量浓度2.5mg/mL，pH=4，上柱速率2mL/min，乙醇体积分数60%，洗脱量为1.7BV~2.0BV.经AB-8大孔树脂分离后，石榴叶多酚的纯度提高到62.44%，再经聚酰胺树脂分离后石榴叶多酚的纯度为92.83%.石榴叶多酚的抗氧化性研究结果表明，3种不同纯度的多酚均具有清除自由基的效果及还原能力，而且纯度越高清除自由基的效果越好，还原能力越强.与芦丁相比，纯化后的石榴叶多酚具有更好的还原力及清除亚硝酸盐的能力.</br>ThemacroporousresinwaschoosenforseparatingcrudepolyphenolsfromtheleavesofPunicagranatumL.，threedifferentpurityofpolyphenolsfromPunicagranatumL.leavesweregot，comparedwithRutin，threedifferentpurityofpolyphenolsfromPunicagranatumL.wereevaluatedwithdifferentantioxidanttests.Theresultsshowedthatwithsamplesolutionconcentrationof2.5mg/mL，pH=4，feedingspeedof2mL/min，ethanolconcentrationof60%，elutionvolumeof1.7BV~2.0BV，thepurityofCPPLwas62.44%，thepurityofpurifiedpolyphenolsofcrudepolyphenolsfromPunicagranatumL.leaveswas92.83%whichwasfurtherimprovedbyPA.TheresultsofoxidationstabilityanalysisofpolyphenolsinPunicagranatumL.leaveswereshowedthatithadthebettereffectinscavengingfreeradicalandreducingpowerwiththehigherpurity，comparedwithrutin，reducingpowerandnitritescavengingactivityofpurifiedpolyphenolsfromPunicagranatumL.leaveswasstronger
%K 石榴叶
%K 多酚
%K 纯化
%K 抗氧化活性&lt
%K /br&gt
%K PunicagranatumL.leaves
%K polyphenols
%K purification
%K antioxidantactivity
%U http://njsfdxzrb.paperonce.org/oa/darticle.aspx?type=view&id=201503012