%0 Journal Article
%T 球形芽孢杆菌BS-10高产培养基的优化<br>Optimization of high yield medium for Bacillus sphaericus strain 10
%A 刘
%A 美
%A 王刘庆
%A 廖美德
%A 等
%J 西北农林科技大学学报（自然科学中文版)
%D 2015
%X 【目的】对杀蚊球形芽孢杆菌BS-10高产培养基进行优化，为该菌株的规模化生产提供理论依据。【方法】以菌株BS-10菌体生物量、干质量和芽孢数为考察指标，对培养基中添加的碳、氮组成成分进行正交试验优化，并对优化所得培养基进行发酵罐扩大培养试验和生物活性测定。【结果】菌株BS-10高产毒力最优培养基（NBFB）配方为胰蛋白胨3 g/L，牛肉膏1 g/L，鱼粉6 g/L，麸皮4 g/L；采用NBFB培养基，可将BS-10培养周期缩短至18 h，芽孢数达4.12×109 CFU/mg，对致倦库蚊幼虫的毒力较基础培养基增大了100倍。【结论】菌株BS-10在NBFB培养基扩大培养中，不仅获得了高产量，而且缩短了发酵周期，具有较好的应用潜力和经济价值。<br>【Objective】The medium for high mosquito larvicide activity of Bacillus sphaericus strain 10 (BS-10) was optimized to guide large-scale production.【Method】In terms of bacterial biomass,dry weight and spore count of strain BS-10,carbon and nitrogen sources of medium were optimized by orthogonal experiment.Then,the biomass,dissolved oxygen,and other related parameters of optimal medium were evaluated through a 7 L fermentor.【Result】 The optimal medium (NBFB) of BS-10 consisted of 3 g/L tryptone,1 g/L beef extract,6 g/L fish meal and 4 g/L wheat bran.Fermentation period was shortened to 18 h with the optimal medium.The maximum spore count was 4.12×109 CFU/mg and the toxicity against Culex quinquefasciatus was increased by 100 times compared to basal medium (NB medium).【Conclusion】Culturing strain BS-10 in NBFB medium obtained higher larvicide activity and shortened the fermentation period,which has a promising application potential and economic value
%K 球形芽孢杆菌
%K 菌株BS-10
%K 致倦库蚊
%K 鱼粉
%K 麸皮&lt
%K br&gt
%K Bacillus sphaericus
%K BS-10
%K Culex quinquefasciatus
%K fish meal
%K wheat bran
%U http://www.xnxbz.net/xbnlkjdxzr/ch/reader/view_abstract.aspx?file_no=20151132&flag=1