%0 Journal Article
%T 泾渭茯茶化学成分HPLC指纹图谱的研究<br>HPLC fingerprints of chemical constituents of Jingwei fu tea
%A 张
%A 宁
%A 宛晓春
%A 周
%A 杰
%A 等
%J 西北农林科技大学学报（自然科学中文版)
%D 2015
%X 【目的】采用高效液相色谱法（HPLC）建立泾渭茯茶化学成分的指纹图谱，以描述泾渭茯茶化学成分的整体特性。【方法】利用HPLC法分析26个泾渭茯茶样品的主要化学成分，通过化学对照品，结合HPLC-MS多级质谱裂解规律，指认泾渭茯茶指纹图谱的主要色谱峰。以乙腈0.4%（体积分数）甲酸水溶液为梯度洗脱系统，在检测波长320 nm、柱温30 ℃、流速1 mL/min条件下，建立泾渭茯茶主要化学成分的指纹图谱。采用中药指纹图谱相似度软件，对泾渭茯茶HPLC指纹图谱进行相似度分析，获取各样品与对照指纹图谱的相似度。【结果】与对照指纹图谱相比，26个不同类型和批次的泾渭茯茶指纹图谱相似度为0.81～0.94，其中共有峰15个。经过HPLC-MS分析，主要的共有峰分别鉴定为没食子酸、可可碱、没食子儿茶素、表没食子儿茶素、儿茶素、表儿茶素、普洱茶素、表儿茶素没食子酸酯和槲皮素。【结论】26个批次的泾渭茯茶样品指纹图谱相似度较高。<br>【Objective】This study investigated the fingerprint of Jingwei fu tea using high performance liquid chromatography (HPLC).【Method】With the gradient elution of HPLC,the main chemical constituents of Jingwei fu tea were analyzed and identified by reference chemicals and HPLC-MS.The gradient elution phase containing acetonitrile and 0.4% formic acid were used to separate the main chemical constituents of Jingwei fu tea at the wavelength of 320 nm,temperature of 30 ℃ and elution ratio of 1 mL/min.The HPLC fingerprints of 26 Jingwei fu tea samples were analyzed by Traditional Chinese medicine (TCM) fingerprint similarity calculation software.【Result】The similarities of 26 samples with different types and batches were in the range of 0.81-0.94 compared with the control HPLC fingerprint generated by TCM fingerprint similarity calculation software.Fifteen shared peaks of HPLC fingerprints were analyzed by HPLC MS.Nine of these peaks were identified as gallic acid,theobromine,gallocatechin,epigallocatechin,catechin,epicatechin,puerins,epicatechin gallate and quercetin.【Conclusion】The fingerprints of 26 Jingwei fu tea samples were highly similar
%K 泾渭茯茶
%K 指纹图谱
%K 化学成分
%K 茶多酚&lt
%K br&gt
%K Jingwei fu tea
%K fingerprint
%K chemical constituents
%K tea polyphenols
%U http://www.xnxbz.net/xbnlkjdxzr/ch/reader/view_abstract.aspx?file_no=20150915&flag=1