%0 Journal Article
%T 外源NO供体硝普钠和镍对玉米生长及抗氧化酶活性的影响<br>Effect of exogenous NO donor SNP and nickel on growth and antioxidant enzymes activities of corn
%A 张忠庆
%A 李韶山
%A 刘金华
%A 等
%J 西北农林科技大学学报（自然科学中文版)
%D 2015
%X 【目的】研究外源NO供体硝普钠(SNP)和镍（Ni）共同作用下玉米幼苗生长及抗氧化酶活性的变化，为镍污染防治提供参考。【方法】以“吉农669”为供试玉米品种，以NO供体SNP和镍处理玉米幼苗，共设6个处理（1.Ni 0 mmol/L+SNP 0 mmol/L，对照；2.Ni 0 mmol/L+SNP 0.05 mmol/L；3.Ni 0 mmol/L+SNP 0.1 mmol/L；4.Ni 1.0 mmol/L+SNP 0 mmol/L；5.Ni 1.0 mmol/L+SNP 0.05 mmol/L；6.Ni 1.0 mmol/L+SNP 0.1 mmol/L），分析各处理下玉米幼苗生长生理指标和抗氧化酶活性的变化。【结果】SNP能够明显缓解由于镍所造成的玉米鲜质量、干质量下降及根系生长受阻的情况；1.0 mmol/L镍（处理4）使玉米植株体内镍含量升高的同时还抑制了铁从玉米根系向地上部分的转移，导致植株地上部铁含量下降，而SNP能够缓解这种抑制作用，使植物体内镍含量降低，铁含量升高。另外，SNP可缓解镍造成的玉米叶片SPAD值下降以及过氧化氢和丙二醛含量升高，增加玉米叶片的超氧化物歧化酶（SOD）、过氧化氢酶(CAT)、抗坏血酸过氧化物酶（APX）和谷胱甘肽过氧化物酶（GPX）等抗氧化酶活性，且高浓度（1.0 mmol/L）SNP效果较好。【结论】外源NO供体SNP能减轻镍对玉米幼苗生长所产生的伤害。<br>【Objective】The growth of maize seedlings and activities of antioxidant enzymes were studied under the interaction of exogenous NO donor SNP and nickel to provide reference for preventing and controlling nickel pollution.【Method】Jinong 669 maize seedlings were treated by NO (SNP) and Ni.The 6 treatments were:1.Ni 0 mmol/L+SNP 0 mmol/L (control),2.Ni 0 mmol/L+SNP 0.05 mmol/L,3.Ni 0 mmol/L+SNP 0.1 mmol/L,4.Ni 1.0 mmol/L+SNP 0 mmol/L,5.Ni 1.0 mmol/L+SNP 0.05 mmol/L,and 6.Ni 1.0 mmol/L+SNP 0.1 mmol/L.The changes of growth and physiological indexes and activities of antioxidant enzymes were analyzed.【Result】SNP significantly relieved the decrease of fresh and dry weights and inhibition of root growth caused by nickel.Nickel with concentration of 1.0 mmol/L (treatment 4) not only increased nickel content in the seedlings,but also inhibited the transfer of iron from corn roots to aboveground parts,which caused the decrease of iron concentration in plant.SNP could relieve this inhibition by decreasing nickel concentration while increasing ion concentration.In addition,SNP alleviated the decrease of SPAD values and the increase of H2O2 and MDA content in corn leaves,and increased the activities of SOD,CAT,APX and GPX.High concentration SNP (1.0 mmol/L) was better.【Conclusion】Exogenous NO donor SNP alleviated the damage caused by nickel in corn seedlings
%K 镍胁迫
%K 玉米
%K 外源一氧化氮
%K 硝普钠
%K 生理特性&lt
%K br&gt
%K nickel stress
%K maize
%K exogenous NO
%K SNP
%K physiological characteristic
%U http://www.xnxbz.net/xbnlkjdxzr/ch/reader/view_abstract.aspx?file_no=20150812&flag=1