%0 Journal Article
%T Determination of Asymmetric Dimethylarginine and Symmetric Dimethylarginine in Biological Samples of Mice Using LC/MS/MS
%A Daisuke Saigusa
%A Mai Takahashi
%A Yoshitomi Kanemitsu
%A Ayako Ishida
%A Takaaki Abe
%A Tohru Yamakuni
%A Naoto Suzuki
%A Yoshihisa Tomioka
%J American Journal of Analytical Chemistry
%P 303-313
%@ 2156-8278
%D 2011
%I Scientific Research Publishing
%R 10.4236/ajac.2011.23038
%X Herein, we present a novel method of asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) determination within biological samples using protein precipitation and LC/MS/MS. Chromatographic separation of ADMA and SDMA was successfully performed using a silica column with optimized elution, or mobile phase, of 10 mM ammonium acetate buffer H<sub>2</sub>O/methanol/acetonitrile (20/30/45, v/v) at pH 4. The calibration ranges were 0.50 ¨C 50.0 ¦Ìg¡ñmL<sup>-1</sup>, and good linearities were obtained for all compounds ( ¦Ã > 0.99). The intra- and inter-assay accuracies with recoveries and precisions at three concentration levels (<i>i.e</i>. 1.00, 5.00 and 25.0 ¦Ìg¡ñmL<sup>-1</sup>) were better than 86.9% and 7.36%, respectively. The analytical performance of the method was evaluated by determination of compounds in plasma, urine and tissues from male BALBc/J mice. For the first time, we were able to characterize the distribution of ADMA, SDMA and ADMA/SDMA in plasma, urine, brain, heart, kidneys, liver, lungs, pancreas and spleen. Additionally, we demonstrated that the ADMA/SDMA ratio in the brain was approximately 10-fold lower than all the other biological samples. Only 10 ¦ÌL of plasma, 1 ¦ÌL of urine and about 25 mg of tissues were required. These results suggest that the developed methodology was useful in ADMA and SDMA determination within biological samples.
%K Asymmetric Dimethylarginine
%K Symmetric Dimethylarginine
%K Creatinine
%K Arginine
%K Tissue
%K Liquid Chromatography/Tandem Mass Spectrometry.
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=5813