%0 Journal Article
%T 单分子荧光共振能量传递及系综生物物理表征人体肌细胞增强因子2d启动子形成的g-四链体
%A 周文华
%A 应立明
%J 物理化学学报
%D 2010
%X dnag-quadruplexes(g4s)arebelievedtobeinvolvedinmanybiologicalprocesses,includingthegeneexpressionregulationandthemaintenanceoftelomere.however,thekineticsofg4,inparticularhowg4formsinthedouble-strandedgenomicdnaisanunderexploredissue.wefoundthataverystableg4canforminthepromoterofhumanmyocyteenhancerfactor2d(mef2d),thegeneencodesatranscriptionfactorbelongingtothemef2(myocyteenhancerfactor-2)familywhichregulatestheresponseofhearttocardiacstresssignals.biophysicalcharacterizations,inparticularsinglemoleculefluorescenceresonanceenergytransfer(smfret)measurements,suggestthatthisg4ismorestablethanitsduplexforminnearphysiologicalconditions.twomajorg4structureswereidentifiedbasedonsinglemoleculeconformationalanalysisontheendandinternallabeleddnasystems.afreeenergylandscapefortheg4hybridizationwasestablishedinlightofthesmfretg4/duplexcompetitionassay.inaddition,theg4hybridizationandunfoldingrateconstantswereobtained.
%K fluorescenceresonanceenergytransfer
%K dnag-quadruplex
%K myocyteenhancerfactor2d
%K unfoldingkinetics
%K singlemolecule
%U http://www.whxb.pku.edu.cn/CN/abstract/abstract26997.shtml