%0 Journal Article
%T Genetically Modified Lactococcus lactis for Delivery of Human Interleukin-10 to Dendritic Cells
%A Inge L. Huibregtse
%A Sebatian A. Zaat
%A Martien L. Kapsenberg
%A Maria A. Sartori da Silva
%A Maikel P. Peppelenbosch
%A Sander J. H. van Deventer
%A Henri Braat
%J Gastroenterology Research and Practice
%D 2012
%I Hindawi Publishing Corporation
%R 10.1155/2012/639291
%X Interleukin-10 (IL-10) plays an indispensable role in mucosal tolerance by programming dendritic cells (DCs) to induce suppressor Th-cells. We have tested the modulating effect of L. lactis secreting human IL-10 (.-10) on DC function in vitro. Monocyte-derived DC incubated with .-10 induced effector Th-cells that markedly suppressed the proliferation of allogenic Th-cells as compared to L. lactis. This suppressive effect was only seen when DC showed increased CD83 and CD86 expression. Furthermore, enhanced production of IL-10 was measured in both .-10-derived DC and Th-cells compared to L. lactis-derived DC and Th-cells. Neutralizing IL-10 during DC-Th-cell interaction and coculturing .-10-derived suppressor Th-cells with allogenic Th-cells in a transwell system prevented the induction of suppressor Th-cells. Only 130 pg/mL of bacterial-derived IL-10 and 40 times more exogenously added recombinant human IL-10 were needed during DC priming for the generation of suppressor Th-cells. The spatially restricted delivery of IL-10 by food-grade bacteria is a promising strategy to induce suppressor Th-cells in vivo and to treat inflammatory diseases.
%U http://www.hindawi.com/journals/grp/2012/639291/