%0 Journal Article
%T Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling
%A Grace O'Maille
%A Eden P. Go
%A Linh Hoang
%A Elizabeth J. Want
%A Colin Smith
%A Paul O'Maille
%A Anders Nordstr m
%A Hirotoshi Morita
%A Chuan Qin
%A Wilasinee Uritboonthai
%A Junefredo Apon
%A Richard Moore
%A James Garrett
%A Gary Siuzdak
%J Spectroscopy: An International Journal
%D 2008
%I Hindawi Publishing Corporation
%R 10.3233/spe-2008-0361
%X Comprehensive detection and quantitation of metabolites from a biological source constitute the major challenges of current metabolomics research. Two chemical derivatization methodologies, butylation and amination, were applied to human serum for ionization enhancement of a broad spectrum of metabolite classes, including steroids and amino acids. LC-ESI-MS analysis of the derivatized serum samples provided a significant signal elevation across the total ion chromatogram to over a 100-fold increase in ionization efficiency. It was also demonstrated that derivatization combined with isotopically labeled reagents facilitated the relative quantitation of derivatized metabolites from individual as well as pooled samples.
%U http://www.hindawi.com/journals/spectroscopy/2008/715905/