%0 Journal Article
%T Applicationofmonoclonalantibodiesagainstchickeninfectiousbursaldiseasevirus(IBDV)
%A 乔忠
%A 詹丽娥
%A 史振心
%A 马丛林
%A 刘王子
%A 宁官宝
%J 华北农学报
%P 135-139
%D 1993
%R 10.7668/hbnxb.1993.S2.024
%X PreliminarystudyhasbeenmadetotestwetherthreestrainsMcAb（1B1,5D6,6D8）areagainstthesameantigendeterminant.ThrougnELISAadditiveandcompetitionexpeiments,itprovedthatthesethreestrainsareagainstdifferentantigendeterminant.Theresultofpositiveserumantigencomponentanalysiswith2strainsIBDVMcAbshowedthatsampleIBDpositiveserumhadobviousinhibitionagainstcombinationof5D6McAbwithcorrespondingantigen.TheresultsofsubstitutionofcorrespondingcomponentinELISAinhibitionexperimentandcompari-sonofnon-IBDserum（SPFchickenserum,ND,MD,IApositiveserum）provedthatIBDanti-serumwastheonlyoneshowinginhibitionagainst5D6McAb.ComparisonwithAGPandelectro-microscopeobservationshowedthatELISAinhibitionexperimentwascharacterisedbyhigh-specificity,rapidityandsensitiveness.799serumsamplesweretestedwithELISAinhibitionanddoubleimmunodiffusion（AGP）experiments.
%K infectiousbursaldisease
%K virus
%K monoclone
%K chicken
%U http://www.hbnxb.net/CN/abstract/abstract6477.shtml