%0 Journal Article
%T Plantsregenerationfromprotoplastandtheirgeneticvariationinfoxtailmillet
%A 赵连元
%A 刁现民
%A 段胜军
%J 华北农学报
%P 35-39
%D 1993
%R 10.7668/hbnxb.1993.S2.006
%X Compactcalliderivedfromimmaturespikeletofafoxtailmilletvariety—Jigu11cann'tbedirectlyusedforprotoplastisolationbecauseofitsfirmphysicalstructure,andmustbeloosenedwithsubculturinginM1,M2andM3mediasuccessivelyandalteringthesemediacompo-sitions.Theloosenedcallicanbeselectedfromtheregulationandusedforprotoplastisolationsuccessfully.RateofprotoplastdivisioninKM8Pmediumwas12.3—33.5%.Calliderivedthroughprotoplastdivisionarelooseandcann'tbeuseddirectlyforplanregenerationbecauseofitssoftphysicalstructure.WhentheyweresubculturedinN6—1,N6—2,N6—3andN6—4media,inwhichthemediacompositionswerechanged,thecompactcalliwereobtainedand129plantletswereregeneratedfromthem.101plants,whichgrewtomaturityaftertransplantingtheplantletsintofield,exhibitedsterilityinsomedegree.Mostofthesubsequentlinesderivedfromtheregen-eratedplantsweresterileandonlytwolinescouldgetnormalreproduction.
%K foxtailmillet(SetariaitalicaBeau.)
%K protoplast
%K plantregeneration
%K geneticvaria-tion
%U http://www.hbnxb.net/CN/abstract/abstract6442.shtml