%0 Journal Article
%T Theprotoplastsisolation,cultureandshootsregenerationofbroccoli(Brassicaoleraceavar.italica)*
%A 王怀名
%A A.Schfer-Menuhr
%A G.Mix-Wagner
%J 华北农学报
%P 28-34
%D 1993
%R 10.7668/hbnxb.1993.S2.005
%X EightF1-hybridcultivarsofbroccoliwerestudied.Weobtainedcelldivision,celledcoloniesandp-calliin5cultivars,rootsandshootsregenerationinonecultivar.Theleavesofpropagatedplantletsinvitrowerecutinto1—2mmpieces,isolatedwithanenzymesolutioncontaining2%cellulaseand1%maceraseonarotaryshaker（50rpm,21℃,3h,2500luxlight）,andpurifiedwitha0.5Msucrosesolution.Thepurifiedprotoplastswereplacedonadropof1%agarose.2—3mlliquidmediumwasaddedaroundtheagarosedrops,andallofthecultureswereincubatedat25℃underlight（4000lux）for16hours.3—5daysafterisolationthecelldivisionwasfound.About7daysafterincubation4multicellularcolonieswereformed.After3—5wksomep-calliweredeveloped.Whenthep-calliwere2—3mmindiameteritwastransferredtoasolidifiedmedium.Oncetheyweredevelopedto1cmindiametertheyweretransferredonare-generationmedium.About5monthsafterincubationsomerootsandshootsgrownfromthecalliwereregen.
%K protoplastsculture
%K shootsregeneration
%K broccoli(Brassicaoleraceavar.italica)
%U http://www.hbnxb.net/CN/abstract/abstract6440.shtml